Linked Life Data

8205232

Source:http://linkedlifedata.com/resource/pubmed/id/8205232

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-7-14
pubmed:abstractText
The use of receptor proteins for biospecific sample handling in liquid and gas chromatography is described. As a model system the uterine estrogen receptor was chosen for the isolation of 17 beta-estradiol and its synthetic agonist diethylstilbestrol. Biochemical characteristics relevant for the use of receptors in sample handling such as the kinetics of receptor-ligand binding, reproducibility and capacity are examined by means of an estrogen radioreceptor assay. Different techniques for the non-covalent immobilization of the estrogen receptor were investigated. Both protamine-coated glass fibre filters and silica particles which bind the receptor via electrostatic interactions have been used for this purpose. The use of the estrogen receptor in the isolation of 17 beta-estradiol and diethylstilbestrol prior to GC-MS analysis is demonstrated and discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
665
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
233-41
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Potential of receptor-ligand interactions for sample handling in liquid and gas chromatography.
pubmed:affiliation
Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University, Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't