|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0020792,
umls-concept:C0020980,
umls-concept:C0029347,
umls-concept:C0035668,
umls-concept:C0042776,
umls-concept:C0205160,
umls-concept:C0205210,
umls-concept:C0331858,
umls-concept:C0370003,
umls-concept:C1524063,
umls-concept:C1704640,
umls-concept:C1706515,
umls-concept:C2347026
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1994-6-30
|
|
pubmed:abstractText |
Influenza A virus infections are a major cause of morbidity and mortality worldwide. Standard diagnostic methods either are not efficient in identifying infected individuals in a timely manner or lack sensitivity. We developed a PCR-enzyme immunoassay (PCR-EIA) for the detection of influenza A virus RNA in respiratory secretions. A reverse transcription PCR was performed with oligonucleotide primers directed at a highly conserved area of the influenza A matrix gene. Amplified DNA was identified by hybridization in solution to a nested biotinylated RNA probe and quantitated in an EIA. PCR-EIA detected small quantities of RNA from the three prevalent subtypes of human influenza A virus. Influenza B and C, parainfluenza, measles, mumps, and respiratory syncytial viruses tested negative. The potential efficiency of PCR-EIA for use in clinical diagnosis was determined by testing 90 nasal wash specimens obtained daily over a 10-day period from nine human volunteers infected with influenza A virus. Thirty-seven of the postinfection samples had detectable influenza A virus RNA by PCR-EIA, whereas only 26 postinfection samples were positive by culture. PCR-EIA was particularly efficient for the identification of influenza A virus in samples obtained more than 4 days after infection. Seventeen of 45 such samples were positive, whereas virus was cultivated from 4 samples (P < 0.00005). All preinfection samples from volunteers subsequently infected with influenza A virus were negative by PCR-EIA, as were samples from a volunteer infected with parainfluenza virus type 3. Nucleic acid amplification techniques represent important tools for the timely and sensitive diagnosis of influenza A virus infections and, therefore, their management and control.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-1320095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-1374080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-1551982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-1572972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-1627644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-1696684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-1703618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-1939505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-2229379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-227920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-2308948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-2403656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-3578619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-3711273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-387816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-4322460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-443244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-5092152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-7462417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8195369-8440931
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0095-1137
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
32
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
623-8
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:8195369-Base Sequence,
pubmed-meshheading:8195369-DNA Primers,
pubmed-meshheading:8195369-Evaluation Studies as Topic,
pubmed-meshheading:8195369-Humans,
pubmed-meshheading:8195369-Immunoenzyme Techniques,
pubmed-meshheading:8195369-Influenza, Human,
pubmed-meshheading:8195369-Influenza A virus,
pubmed-meshheading:8195369-Molecular Sequence Data,
pubmed-meshheading:8195369-Nasal Mucosa,
pubmed-meshheading:8195369-Polymerase Chain Reaction,
pubmed-meshheading:8195369-RNA, Viral,
pubmed-meshheading:8195369-Sensitivity and Specificity
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
Use of PCR-enzyme immunoassay for identification of influenza A virus matrix RNA in clinical samples negative for cultivable virus.
|
|
pubmed:affiliation |
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
