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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1994-6-9
|
| pubmed:abstractText |
Transforming growth factor-beta (TGF-beta) is a potent regulator of cellular differentiation, proliferation, migration, and protein expression. These properties have been exploited to create a variety of bioassays for detecting the mature growth factor. In this paper, we describe a highly sensitive and specific, nonradioactive quantitative bioassay for TGF-beta based on its ability to induce plasminogen activator inhibitor-1 (PAI-1) expression. Mink lung epithelial cells (MLEC) were stably transfected with an expression construct containing a truncated PAI-1 promoter fused to the firefly luciferase reporter gene. Addition of TGF-beta (0.2 to > 30 pM) to the transfectants resulted in a dose-dependent increase in luciferase activity in the cell lysates. Although responsive to TGF-beta, this promoter fragment was only minimally influenced by other known inducers of PAI-1 expression. When compared to the widely used MLEC assay, this assay demonstrated greater sensitivity and specificity, allowing quantification of TGF-beta in complex biological solutions.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Plasminogen Activator Inhibitor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0003-2697
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
216
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
276-84
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8179182-Animals,
pubmed-meshheading:8179182-CHO Cells,
pubmed-meshheading:8179182-Cricetinae,
pubmed-meshheading:8179182-Epithelial Cells,
pubmed-meshheading:8179182-Epithelium,
pubmed-meshheading:8179182-Growth Substances,
pubmed-meshheading:8179182-Guinea Pigs,
pubmed-meshheading:8179182-HeLa Cells,
pubmed-meshheading:8179182-Humans,
pubmed-meshheading:8179182-Luciferases,
pubmed-meshheading:8179182-Lung,
pubmed-meshheading:8179182-Mink,
pubmed-meshheading:8179182-Plasminogen Activator Inhibitor 1,
pubmed-meshheading:8179182-Promoter Regions, Genetic,
pubmed-meshheading:8179182-Radioligand Assay,
pubmed-meshheading:8179182-Recombinant Fusion Proteins,
pubmed-meshheading:8179182-Sensitivity and Specificity,
pubmed-meshheading:8179182-Transfection,
pubmed-meshheading:8179182-Transforming Growth Factor beta
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
An assay for transforming growth factor-beta using cells transfected with a plasminogen activator inhibitor-1 promoter-luciferase construct.
|
| pubmed:affiliation |
Department of Cell Biology, New York University Medical Center, New York.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|