Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
1994-6-6
pubmed:abstractText
Somatostatin has a modulatory role in regulating the membrane conductance in hippocampal neurons. To examine the signal transducing molecules involved in this process, we isolated the cDNA encoding the dominant rat hippocampal somatostatin receptor, SSTR4. Distribution of SSTR4 in the adult central nervous system was restricted to the hippocampus, cerebral cortex, striatum, hypothalamus, and thalamus, as determined by Northern blot analysis and in situ hybridization. In SSTR4-expressing Chinese hamster ovary cells, SSTR4 was functionally coupled not only to inhibition of adenylate cyclase, but also to activation of both arachidonate release and mitogen-activated protein (MAP) kinase cascade, with similar ED50 values. All of these pathways, including both MAP kinase kinase and MAP kinase activation, were completely blocked by pretreatment with pertussis toxin. On the other hand, neither inositol 1,4,5-trisphosphate synthesis nor intracellular Ca2+ mobilization was induced upon SSTR4 stimulation. These data indicate that the hippocampal functions of somatostatin might be mediated through diverse but selective second messenger systems activated via SSTR4 and reveal an unsuspected coupling of a neuronal SSTR subtype to a mitogenic signaling pathway. SSTR4, in addition, provides a useful system to study the Ca(2+)-independent, Gi-dependent (pertussis toxin-sensitive) pathway of MAP kinase activation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase, http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase Toxin, http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary, http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates, http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1, http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Somatostatin, http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
269
pubmed:geneSymbol
SSTR
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12722-30
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8175684-Adenylate Cyclase, pubmed-meshheading:8175684-Adenylate Cyclase Toxin, pubmed-meshheading:8175684-Animals, pubmed-meshheading:8175684-Arachidonic Acid, pubmed-meshheading:8175684-Base Sequence, pubmed-meshheading:8175684-CHO Cells, pubmed-meshheading:8175684-Calcium, pubmed-meshheading:8175684-Cloning, Molecular, pubmed-meshheading:8175684-Cricetinae, pubmed-meshheading:8175684-DNA, Complementary, pubmed-meshheading:8175684-Enzyme Activation, pubmed-meshheading:8175684-GTP-Binding Proteins, pubmed-meshheading:8175684-Hippocampus, pubmed-meshheading:8175684-Inositol Phosphates, pubmed-meshheading:8175684-Male, pubmed-meshheading:8175684-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:8175684-Molecular Sequence Data, pubmed-meshheading:8175684-Pertussis Toxin, pubmed-meshheading:8175684-Protein-Serine-Threonine Kinases, pubmed-meshheading:8175684-Protein-Tyrosine Kinases, pubmed-meshheading:8175684-Rats, pubmed-meshheading:8175684-Rats, Sprague-Dawley, pubmed-meshheading:8175684-Receptors, Somatostatin, pubmed-meshheading:8175684-Signal Transduction, pubmed-meshheading:8175684-Virulence Factors, Bordetella
pubmed:year
1994
pubmed:articleTitle
Functional coupling of SSTR4, a major hippocampal somatostatin receptor, to adenylate cyclase inhibition, arachidonate release and activation of the mitogen-activated protein kinase cascade.
pubmed:affiliation
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't