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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1994-6-2
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pubmed:abstractText |
We report for the first time that, after centrifugation of adult bovine vitreous, the hyaluronan-rich supernatant contains collagens which can be isolated in their intact forms by precipitation with 4.5 M NaCl. This precipitate constituted approx. 4% of the total vitreous collagen and comprised collagen types IX and II (in the approximate ratio of 4:1) with negligible amounts of type-V/XI collagen. Type-II collagen was present partly in a pro-alpha 1(II) form, suggesting that there is active synthesis of type-II collagen into the matrix of adult bovine vitreous. Type-IX collagen was purified (2-2.5 mg/l of vitreous) and its glycosaminoglycan chain composition was analysed. Bovine vitreous type-IX collagen always possessed a glycosaminoglycan chain of comparatively low M(r) that was predominantly 4-sulphated, with chondroitin 6-sulphate representing a more minor component. By contrast, chick vitreous has been shown to contain type-IX collagen which always possesses a high-M(r) chondroitin sulphate chain that is predominantly 6-sulphated. The functional significance of these different glycosaminoglycan chain lengths and sulphation patterns is discussed.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-1289129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-13786180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-13971270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-1400327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-1425674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-1429648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-1572350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-1577766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-1577778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-1599476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-1631157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-1729132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-1898337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-1909507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-2002024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-2012600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-2016136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-2059551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-2204435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-2209617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-2324108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-2463256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-2511833,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-5432063,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-6424501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-6721835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-7126595,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8172611-884145
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
299 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
497-505
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8172611-Animals,
pubmed-meshheading:8172611-Blotting, Western,
pubmed-meshheading:8172611-Carbohydrate Sequence,
pubmed-meshheading:8172611-Cattle,
pubmed-meshheading:8172611-Chickens,
pubmed-meshheading:8172611-Chromatography, DEAE-Cellulose,
pubmed-meshheading:8172611-Chromatography, Gel,
pubmed-meshheading:8172611-Collagen,
pubmed-meshheading:8172611-Disaccharides,
pubmed-meshheading:8172611-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8172611-Glycosaminoglycans,
pubmed-meshheading:8172611-Hydroxyproline,
pubmed-meshheading:8172611-Indicators and Reagents,
pubmed-meshheading:8172611-Molecular Sequence Data,
pubmed-meshheading:8172611-Molecular Weight,
pubmed-meshheading:8172611-Vitreous Body
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pubmed:year |
1994
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pubmed:articleTitle |
Extraction and characterization of the tissue forms of collagen types II and IX from bovine vitreous.
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pubmed:affiliation |
School of Biological Sciences, University of Manchester, U.K.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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