Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1994-6-1
pubmed:abstractText
We performed experiments to determine whether nitric oxide promoted the formation of intracellular S-nitrosothiol adducts in human neutrophils. At concentrations sufficient to inhibit chemoattractant-induced superoxide anion production, nitric oxide caused a depletion of measurable intracellular glutathione as determined by both the monobromobimane HPLC method and the glutathione reductase recycling assay. The depletion of glutathione could be shown to be due to the formation of intracellular S-nitrosoglutathione as indicated by the ability of sodium borohydride treatment of cytosol to result in the complete recovery of measurable glutathione. The formation of intracellular S-nitrosylated compounds was confirmed by the capacity of cytosol derived from nitric oxide-treated cells to ADP-ribosylate glyceraldehyde-3-phosphate dehydrogenase. Depletion of intracellular glutathione was accompanied by a rapid and concomitant activation of the hexose monophosphate shunt (HMPS) following exposure to nitric oxide. Kinetic studies demonstrated that nitric oxide-dependent activation of the HMPS was reversible and paralleled nitric oxide-induced glutathione depletion. Synthetic preparations of S-nitrosoglutathione shared with nitric oxide the capacity to inhibit superoxide anion production and activate the HMPS. These data suggest that nitric oxide may regulate cellular functions via the formation of intracellular S-nitrosothiol adducts and the activation of the HMPS.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-1281150, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-1281928, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-1325644, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-1325992, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-1379614, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-1443537, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-14474846, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-1645341, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-1675786, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-1711326, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-1898602, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-2077935, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-2322590, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-2784476, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-4388022, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-6137189, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-7304929, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-7687412, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-8216401, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-8327504, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-8343180, http://linkedlifedata.com/resource/pubmed/commentcorrection/8170969-8466523
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Diphosphate Ribose, http://linkedlifedata.com/resource/pubmed/chemical/Cysteine, http://linkedlifedata.com/resource/pubmed/chemical/Glutathione, http://linkedlifedata.com/resource/pubmed/chemical/Glyceraldehyde-3-Phosphate..., http://linkedlifedata.com/resource/pubmed/chemical/N-Formylmethionine..., http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide, http://linkedlifedata.com/resource/pubmed/chemical/Nitroprusside, http://linkedlifedata.com/resource/pubmed/chemical/Nitroso Compounds, http://linkedlifedata.com/resource/pubmed/chemical/S-Nitrosoglutathione, http://linkedlifedata.com/resource/pubmed/chemical/S-Nitrosothiols, http://linkedlifedata.com/resource/pubmed/chemical/S-nitrosocysteine, http://linkedlifedata.com/resource/pubmed/chemical/Superoxides
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3680-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Nitric oxide reacts with intracellular glutathione and activates the hexose monophosphate shunt in human neutrophils: evidence for S-nitrosoglutathione as a bioactive intermediary.
pubmed:affiliation
Department of Medicine, New York University Medical Center, NY 10003.
pubmed:publicationType
Journal Article, In Vitro