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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1994-6-1
|
| pubmed:abstractText |
Insulin-like growth factor-II (IGF-II) modulates myogenesis in muscle cell cultures, in utero. IGF-II gene expression is developmentally regulated in several tissues including muscle. Determining whether IGF-II is expressed by developing muscle cells or by neighbouring cells in developing muscle tissue is crucial for determining whether IGF-II exerts a paracrine or an autocrine affect on myogenesis. Semitendinosus muscle samples from 12 bovine fetuses ranging from 60 to 274 days post conception (pc) were analysed for the amount and localization of muscle IGF-II mRNA using Northern, dot blot and in situ hybridization analyses. Northern blot analysis revealed multiple IGF-II transcripts of 5.1, 3.7, 2.6, 2.0, 1.7 and 1.1 kb in developing bovine muscle tissue. The relative amount of muscle IGF-II mRNA increased (P < 0.05) until 162 days pc, then decreased (P < 0.01) to near undetectable levels by the end of gestation (approximately 284 days pc). Between 60 and 162 days pc, in situ hybridization revealed that the majority of the IGF-II transcripts were localized to developing muscle cells rather than connective tissue. After 162 days pc the IGF-II hybridization signal shifted away from muscle cells and greater accumulation was observed in the connective tissue at 274 days pc. These data confirm that the expression of IGF-II in developing bovine muscle tissue is primarily localized in muscle cells and support the claim that IGF-II acts as an autocrine-acting growth factor during myogenesis in vivo.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0022-0795
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
140
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
179-87
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8169553-Animals,
pubmed-meshheading:8169553-Blotting, Northern,
pubmed-meshheading:8169553-Cattle,
pubmed-meshheading:8169553-Immunoblotting,
pubmed-meshheading:8169553-In Situ Hybridization,
pubmed-meshheading:8169553-Insulin-Like Growth Factor II,
pubmed-meshheading:8169553-Liver,
pubmed-meshheading:8169553-Muscles,
pubmed-meshheading:8169553-RNA, Messenger,
pubmed-meshheading:8169553-RNA Probes
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
In situ localization of muscle insulin-like growth factor-II mRNA in developing bovine fetuses.
|
| pubmed:affiliation |
Laboratoire Croissance et Métabolisme des Herbivores, INRA, St-Genes Champagnelle, France.
|
| pubmed:publicationType |
Journal Article
|