Linked Life Data

8150920

Source:http://linkedlifedata.com/resource/pubmed/id/8150920

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1994-5-12
pubmed:abstractText
Calcium has an important role in the events of egg activation and early preimplantation development. We investigated changes in intracellular calcium concentration in human oocytes at fertilization using the calcium-sensitive photoprotein aequorin. Oocytes were donated for research by patients undergoing in-vitro fertilization treatment in the Department of Obstetrics and Gynaecology. Cumulus cells, and in some cases zonae pellucidae, were removed by appropriate enzyme treatment. Single oocytes were micro-injected with aequorin and incubated in a chamber perfused with pre-equilibrated culture medium in a photomultiplier system. Eleven zona-intact and 15 zona-free oocytes were incubated with sperm, and oocytes from each group were incubated without sperm as controls. Dramatic transient increases in intracellular free calcium concentration were recorded in three zona-intact and seven zona-free oocytes, thought to be the first direct measurements of intracellular changes in human oocytes at fertilization. The amplitude (up to 2.5 microM), duration (120 s) and frequency (every 10-35 min) of these transients were similar in zona-intact and zona-free oocytes. They resemble those recorded in mouse oocytes, which may therefore be a suitable model for biochemical events at human fertilization.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0268-1161
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2174-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Oscillations in intracellular free calcium induced by spermatozoa in human oocytes at fertilization.
pubmed:affiliation
Department of Obstetrics and Gynaecology, University of Liverpool, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't