Linked Life Data

8123725

Source:http://linkedlifedata.com/resource/pubmed/id/8123725

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11-12
pubmed:dateCreated
1994-4-14
pubmed:abstractText
We have developed a fully automated on-line extraction-purification method for peptide leukotrienes in biological fluids using a column-switching technique. Individual leukotrienes in HPLC-collected fractions are determined by immunoassay techniques. Leukotrienes were extracted from nasal lavage samples and eluted from the solid-phase extraction cartridge into the HPLC column with methanol-ammonium acetate buffer (60:40, v/v) pH 5.4, as a mobile phase. This method provides good recoveries, excellent resolution of leukotrienes and is suitable to be combined with off-line radioimmunoassay (RIA) or enzymoimmunoassay (EIA). The most important losses are observed after evaporation-concentration and lyophilization. We also have compared RIA with EIA determination. Significant differences are found between RIA values and EIA values.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1135-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Application of totally automated on-line sample clean up system for extraction and high-performance liquid chromatography separation of peptide leukotrienes.
pubmed:affiliation
Molecular Pathology Unit, Centro de Investigación y Desarrollo, CSIC, Barcelona, Spain.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't