Linked Life Data

8117277

Source:http://linkedlifedata.com/resource/pubmed/id/8117277

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-3-29
pubmed:abstractText
In contrast with many physiological studies suggesting that histamine H2 receptors are present on acid-secreting parietal cells of the gastric epithelium, it was recently shown that immune cells in the lamina propria are the only cells expressing H2-receptor mRNAs (Mezey and Palkovits, Science, 1992, 258, 1662-1665). We have reinvestigated the cellular localization of H2 receptors in the rat stomach by visualizing both the H2 receptor mRNA and the H2-receptor protein itself. In situ hybridization histochemistry performed with an antisense riboprobe for the rat H2 receptor, and autoradiographic distribution of 125I-aminopotentidine binding sites, a highly selective H2-receptor ligand, did not show any labeling of the lamina propria. Signals were clearly and solely detected in the gastric epithelium, the strongest being observed in the upper part of the glands where the H2 receptor gene transcripts were only detected within parietal cells. In situ hybridization performed with an antisense riboprobe for L-histidine decarboxylase mRNA confirmed the basal localization of the histamine-synthetizing cells in the rat gastric gland, at some distance from parietal histamine-sensitive cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
198
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1195-202
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Localization of the histamine H2 receptor and gene transcripts in rat stomach: back to parietal cells.
pubmed:affiliation
Unité de Neurobiologie et Pharmacologie (U. 109) de l'INSERM, Centre Paul Broca, Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't