pubmed-article:8095156 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:8095156 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
pubmed-article:8095156 | lifeskim:mentions | umls-concept:C0026844 | lld:lifeskim |
pubmed-article:8095156 | lifeskim:mentions | umls-concept:C1135918 | lld:lifeskim |
pubmed-article:8095156 | lifeskim:mentions | umls-concept:C0063695 | lld:lifeskim |
pubmed-article:8095156 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
pubmed-article:8095156 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
pubmed-article:8095156 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
pubmed-article:8095156 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
pubmed-article:8095156 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:8095156 | pubmed:dateCreated | 1993-4-8 | lld:pubmed |
pubmed-article:8095156 | pubmed:abstractText | Human atherosclerotic plaques contain numerous smooth muscle cells (SMCs) that express intercellular adhesion molecule-1 (ICAM-1). Expression of ICAM-1 in different cells is known to be regulated by tumor necrosis factor-alpha (TNF-alpha), which has recently been found to be present in the intimal thickening of human arteries. Therefore, we studied the effect of TNF-alpha on ICAM-1 mRNA content and surface expression in cultured human aortic SMCs by using the methods of Northern blotting and immunofluorescence flow cytometry. Under basal conditions of cultivation, ICAM-1 mRNA was not revealed in SMCs. However, treatment of the cells with recombinant human TNF-alpha induced substantial levels of ICAM-1 mRNA. The content of ICAM-1 on the surface of SMCs also increased in a dose- and time-dependent manner after incubation with TNF-alpha. Twenty-four hours of treatment with 10 ng/mL TNF-alpha led to an approximately 10-fold increase in ICAM-1 surface expression in the SMCs. Under the same conditions, pretreatment of SMCs with TNF-alpha resulted in a twofold increase of their adhesiveness for monocytes. In the presence of anti-ICAM-1 monoclonal antibody 10F3, monocyte adhesion to TNF-alpha-pretreated SMCs was significantly inhibited, suggesting that the observed monocyte-SMC interaction involved the ICAM-1 expressed on SMC surfaces as a result of TNF-alpha stimulation. These results led us to propose that TNF-alpha may act a regulator of functional ICAM-1 expression on the SMC surface and thus can increase the possibility of interactions between mononuclear cells and SMCs in atherosclerotic plaques. | lld:pubmed |
pubmed-article:8095156 | pubmed:language | eng | lld:pubmed |
pubmed-article:8095156 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8095156 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:8095156 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8095156 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8095156 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8095156 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8095156 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8095156 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8095156 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:8095156 | pubmed:month | Mar | lld:pubmed |
pubmed-article:8095156 | pubmed:issn | 1049-8834 | lld:pubmed |
pubmed-article:8095156 | pubmed:author | pubmed-author:MoreauCC | lld:pubmed |
pubmed-article:8095156 | pubmed:author | pubmed-author:LamaziereJ... | lld:pubmed |
pubmed-article:8095156 | pubmed:author | pubmed-author:BonnetJJ | lld:pubmed |
pubmed-article:8095156 | pubmed:author | pubmed-author:CouffinhalTT | lld:pubmed |
pubmed-article:8095156 | pubmed:author | pubmed-author:DuplàaCC | lld:pubmed |
pubmed-article:8095156 | pubmed:author | pubmed-author:LabatLL | lld:pubmed |
pubmed-article:8095156 | pubmed:author | pubmed-author:PrintsevaOO | lld:pubmed |
pubmed-article:8095156 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:8095156 | pubmed:volume | 13 | lld:pubmed |
pubmed-article:8095156 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:8095156 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:8095156 | pubmed:pagination | 407-14 | lld:pubmed |
pubmed-article:8095156 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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pubmed-article:8095156 | pubmed:meshHeading | pubmed-meshheading:8095156-... | lld:pubmed |
pubmed-article:8095156 | pubmed:year | 1993 | lld:pubmed |
pubmed-article:8095156 | pubmed:articleTitle | Tumor necrosis factor-alpha stimulates ICAM-1 expression in human vascular smooth muscle cells. | lld:pubmed |
pubmed-article:8095156 | pubmed:affiliation | Institut National de la Santé et de la Recherche Médicale (INSERM) Unit 8 de Cardiologie, Pessac, France. | lld:pubmed |
pubmed-article:8095156 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:8095156 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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