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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1994-10-14
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pubmed:abstractText |
Previous studies showed that CCAAT/enhancer binding protein alpha (C/EBP alpha) is required for differentiation of 3T3-L1 preadipocytes induced by exogenous hormonal agents. It was not possible to ascertain, however, whether C/EBP alpha alone is sufficient to induce differentiation because its antimitogenic activity precluded propagating 3T3-L1 cell lines that constitutively express C/EBP alpha at high levels. This problem was circumvented by using 3T3-L1 preadipocytes stably transfected with an isopropyl beta-D-thiogalactoside (IPTG)-inducible p42 C/EBP alpha expression vector system. IPTG-induced expression of the 42-kDa isoform of C/EBP alpha in preadipocytes caused expression of several endogenous adipocyte-specific genes (genes encoding the 422 adipose P2 protein, glucose transporter 4, and C/EBP alpha) and the accumulation of cytoplasmic triglyceride. Thus, C/EBP alpha is not only necessary but also is sufficient to trigger differentiation of growth-arrested 3T3-L1 preadipocytes without use of exogenous hormonal agents.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-10298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-1321521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-1373117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-1566086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-1638118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-165899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-1681537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-1846704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-1935900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-1987644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-2006196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-2253873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-2404278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-2511432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-2606350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-2654938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-2792758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-3943125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-3968175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-3988766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-6206497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-6928620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-701249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-7372608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-7946535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-8346250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-8367486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-8415748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-8493090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-92801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8090719-949738
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8757-61
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8090719-3T3 Cells,
pubmed-meshheading:8090719-Adipocytes,
pubmed-meshheading:8090719-Animals,
pubmed-meshheading:8090719-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:8090719-Cell Differentiation,
pubmed-meshheading:8090719-DNA-Binding Proteins,
pubmed-meshheading:8090719-Enhancer Elements, Genetic,
pubmed-meshheading:8090719-Gene Expression Regulation,
pubmed-meshheading:8090719-Mice,
pubmed-meshheading:8090719-Nuclear Proteins,
pubmed-meshheading:8090719-RNA, Messenger,
pubmed-meshheading:8090719-Transcription Factors
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pubmed:year |
1994
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pubmed:articleTitle |
CCAAT/enhancer binding protein alpha is sufficient to initiate the 3T3-L1 adipocyte differentiation program.
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pubmed:affiliation |
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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