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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1994-9-16
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pubmed:abstractText |
A latent MAP kinase kinase kinase activity previously detected after chromatography of PC12 cell extracts on Mono Q [(1992) FEBS Lett. 314, 461-465] has been identified as B-Raf by immunoblotting and immunoprecipitation experiments and by its specific activation with B-Raf antibodies. B-Raf is phosphorylated after stimulation of PC12 cells with nerve growth factor or epidermal growth factor, but this is not accompanied by activation of the Mono Q-purified enzyme whether assayed in the absence or presence of GTP-Ras.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
350
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8062910-Amino Acid Sequence,
pubmed-meshheading:8062910-Animals,
pubmed-meshheading:8062910-Blotting, Western,
pubmed-meshheading:8062910-Chromatography, Ion Exchange,
pubmed-meshheading:8062910-Enzyme Activation,
pubmed-meshheading:8062910-MAP Kinase Kinase Kinases,
pubmed-meshheading:8062910-Molecular Sequence Data,
pubmed-meshheading:8062910-PC12 Cells,
pubmed-meshheading:8062910-Phosphorylation,
pubmed-meshheading:8062910-Precipitin Tests,
pubmed-meshheading:8062910-Protein-Serine-Threonine Kinases,
pubmed-meshheading:8062910-Proto-Oncogene Proteins,
pubmed-meshheading:8062910-Proto-Oncogene Proteins c-raf,
pubmed-meshheading:8062910-Rats
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pubmed:year |
1994
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pubmed:articleTitle |
Identification of a latent MAP kinase kinase kinase in PC12 cells as B-raf.
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pubmed:affiliation |
Department of Biochemistry, University of Dundee, Scotland, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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