| pubmed-article:8061622 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8061622 | lifeskim:mentions | umls-concept:C0040669 | lld:lifeskim |
| pubmed-article:8061622 | lifeskim:mentions | umls-concept:C2717940 | lld:lifeskim |
| pubmed-article:8061622 | lifeskim:mentions | umls-concept:C0184661 | lld:lifeskim |
| pubmed-article:8061622 | lifeskim:mentions | umls-concept:C0220825 | lld:lifeskim |
| pubmed-article:8061622 | lifeskim:mentions | umls-concept:C0683598 | lld:lifeskim |
| pubmed-article:8061622 | lifeskim:mentions | umls-concept:C1516048 | lld:lifeskim |
| pubmed-article:8061622 | lifeskim:mentions | umls-concept:C0052009 | lld:lifeskim |
| pubmed-article:8061622 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:8061622 | pubmed:dateCreated | 1994-9-22 | lld:pubmed |
| pubmed-article:8061622 | pubmed:abstractText | Three commonly used transfection techniques (electroporation, calcium phosphate precipitation and scrape loading) and a novel procedure combining the latter two methods were evaluated and conditions optimised for successful transfection of human HepG2 cells with plasmid DNA incorporating a mouse MHC Class I gene and a selectable marker (neomycin transferase gene) conferring resistance to G418. While transfection with linear DNA by scrape-loading gave satisfactory results, transfer of cloned circular DNA by electroporation, calcium phosphate precipitation or a combined use of scrape-loading and calcium phosphate gave best results for HepG2 cells. | lld:pubmed |
| pubmed-article:8061622 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8061622 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061622 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8061622 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061622 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061622 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061622 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061622 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061622 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061622 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061622 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061622 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061622 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061622 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061622 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061622 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8061622 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8061622 | pubmed:issn | 1039-9712 | lld:pubmed |
| pubmed-article:8061622 | pubmed:author | pubmed-author:KumarSS | lld:pubmed |
| pubmed-article:8061622 | pubmed:author | pubmed-author:LundTT | lld:pubmed |
| pubmed-article:8061622 | pubmed:author | pubmed-author:DunckleyM GMG | lld:pubmed |
| pubmed-article:8061622 | pubmed:author | pubmed-author:O'DowdCC | lld:pubmed |
| pubmed-article:8061622 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8061622 | pubmed:volume | 32 | lld:pubmed |
| pubmed-article:8061622 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8061622 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8061622 | pubmed:pagination | 1059-66 | lld:pubmed |
| pubmed-article:8061622 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:meshHeading | pubmed-meshheading:8061622-... | lld:pubmed |
| pubmed-article:8061622 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8061622 | pubmed:articleTitle | A comparative evaluation of three transfection procedures as assessed by resistance to G418 conferred to HEPG2 cells. | lld:pubmed |
| pubmed-article:8061622 | pubmed:affiliation | Tenovus Laboratory, Faculty of Medicine, Southampton University Hospitals, England. | lld:pubmed |
| pubmed-article:8061622 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8061622 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:8061622 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
