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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1994-9-22
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pubmed:abstractText |
Three commonly used transfection techniques (electroporation, calcium phosphate precipitation and scrape loading) and a novel procedure combining the latter two methods were evaluated and conditions optimised for successful transfection of human HepG2 cells with plasmid DNA incorporating a mouse MHC Class I gene and a selectable marker (neomycin transferase gene) conferring resistance to G418. While transfection with linear DNA by scrape-loading gave satisfactory results, transfer of cloned circular DNA by electroporation, calcium phosphate precipitation or a combined use of scrape-loading and calcium phosphate gave best results for HepG2 cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Circular,
http://linkedlifedata.com/resource/pubmed/chemical/Gentamicins,
http://linkedlifedata.com/resource/pubmed/chemical/Kanamycin Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotransferases (Alcohol Group...,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-tricalcium phosphate,
http://linkedlifedata.com/resource/pubmed/chemical/antibiotic G 418,
http://linkedlifedata.com/resource/pubmed/chemical/calcium phosphate,
http://linkedlifedata.com/resource/pubmed/chemical/dicalcium phosphate anhydrous,
http://linkedlifedata.com/resource/pubmed/chemical/monocalcium phosphate,
http://linkedlifedata.com/resource/pubmed/chemical/tetracalcium phosphate
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1039-9712
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1059-66
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8061622-Animals,
pubmed-meshheading:8061622-Calcium Phosphates,
pubmed-meshheading:8061622-Cloning, Molecular,
pubmed-meshheading:8061622-DNA,
pubmed-meshheading:8061622-DNA, Circular,
pubmed-meshheading:8061622-Drug Resistance,
pubmed-meshheading:8061622-Electroporation,
pubmed-meshheading:8061622-Genes, MHC Class I,
pubmed-meshheading:8061622-Gentamicins,
pubmed-meshheading:8061622-Humans,
pubmed-meshheading:8061622-Kanamycin Kinase,
pubmed-meshheading:8061622-Mice,
pubmed-meshheading:8061622-Phosphotransferases (Alcohol Group Acceptor),
pubmed-meshheading:8061622-Plasmids,
pubmed-meshheading:8061622-Transfection,
pubmed-meshheading:8061622-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
A comparative evaluation of three transfection procedures as assessed by resistance to G418 conferred to HEPG2 cells.
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pubmed:affiliation |
Tenovus Laboratory, Faculty of Medicine, Southampton University Hospitals, England.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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