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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1977-8-25
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pubmed:abstractText |
The use of fluorescence recovery after photobleaching (FRAP) techniques to monitor the lateral mobility of plant lectin-receptor complexes on the surface of single, living mammalian cells is described in detail. FRAP measurements indicate that over 75% of the wheat germ agglutinin receptor (WGA-receptor) complexes on the surface of human embryo fibroblasts are mobile. These WGS-receptor complexes diffuse laterally (as opposed to flow) on the cell surface with a diffusion coefficient in the range of 2 X 10(-11) to 2 X 10(-10) cm2/sec. Both the percentage of mobile WGA-receptor complexes and the mean diffusion coefficient of these complexes are higher than that obtained from earlier FRAP measurements of the mobility of concanavalin A-receptor (Con A-receptor) complexes in a variety of cell types. The possible reasons for the differing mobilities of WGA and Con A receptors are discussed.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0091-7419
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
565(417)-576(428)
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:800621-Cell Line,
pubmed-meshheading:800621-Cell Membrane,
pubmed-meshheading:800621-Cytological Techniques,
pubmed-meshheading:800621-Diffusion,
pubmed-meshheading:800621-Fluorescence,
pubmed-meshheading:800621-Humans,
pubmed-meshheading:800621-Lasers,
pubmed-meshheading:800621-Lectins,
pubmed-meshheading:800621-Light,
pubmed-meshheading:800621-Male,
pubmed-meshheading:800621-Microscopy, Fluorescence,
pubmed-meshheading:800621-Models, Biological,
pubmed-meshheading:800621-Plant Lectins,
pubmed-meshheading:800621-Receptors, Concanavalin A,
pubmed-meshheading:800621-Receptors, Drug,
pubmed-meshheading:800621-Triticum
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pubmed:year |
1976
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pubmed:articleTitle |
Measurement of the lateral mobility of cell surface components in single, living cells by fluorescence recovery after photobleaching.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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