Linked Life Data

8006030

Source:http://linkedlifedata.com/resource/pubmed/id/8006030

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
25
pubmed:dateCreated
1994-7-20
pubmed:abstractText
Secretion of catecholamines from individual bovine adrenal medullary cells in culture was examined by amperometry with 1-micron radius carbon-fiber electrodes. Vesicular secretion is observed as a series of current spikes upon exposure to a secretagogue. The small size of the electrodes was exploited to map exocytotic release sites on the surface of bovine adrenal medullary cells. These studies reveal for the first time that release sites are spatially localized on endocrine cells in culture for a time scale of at least 15 min. Fluorescent monitoring by confocal microscopy of deposition of dopamine-beta-hydroxylase from the vesicular membrane into the plasma membrane during exocytosis confirms the existence of zones of exocytotic inactivity on the surface of the cell. Measurement of coincident spikes with two adjacent electrodes (2.5-microns radius) has allowed the spatial resolution for measurement of exocytosis to be defined as 2 microns from the projected circumference of the electrode on the surface of the cell. In this small domain, point-source release and diffusional broadening would result in narrow spikes (Schroeder, T. J., Jankowski, J. A., Kawagoe, K. T., Wightman, R. M., Lefrou, C., and Amatore, C. (1992) Anal. Chem. 64, 3077-3083), a feature not seen in the data. Thus, for adrenal medullary cells, release following vesicular fusion is not instantaneous, but is a prolonged event occurring over several milliseconds.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
269
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17215-20
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Zones of exocytotic release on bovine adrenal medullary cells in culture.
pubmed:affiliation
Department of Chemistry, University of North Carolina at Chapel Hill 27599-3290.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't