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rdf:type |
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lifeskim:mentions |
umls-concept:C0012472,
umls-concept:C0016030,
umls-concept:C0017262,
umls-concept:C0021755,
umls-concept:C0021760,
umls-concept:C0069535,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0205263,
umls-concept:C1704675,
umls-concept:C2911684
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pubmed:issue |
1
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pubmed:dateCreated |
1994-7-15
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pubmed:abstractText |
The role of Oncostatin M (OM), a monocyte/macrophage and T-cell product, in regulating IL-6 expression in fibroblasts of lung or synovial origin was examined in vitro. Although by itself OM had a minimal effect on enhancing IL-6 production by fibroblasts, in combination with IL-1 alpha or PGE2, OM addition resulted in a dose-dependent synergistic enhancement of IL-6 production. This synergistic effect with either IL-1 alpha (5 ng/ml) or PGE2 (10(-7) M) was clearly evident at concentrations of OM of 10, 20 or 50 ng/ml. Levels of IL-6 resulting from OM and IL-1 alpha stimulation could be reduced by indomethacin (10(-6) M) and restored again by also adding PGE2. Northern blots probed for IL-6 mRNA showed cooperative enhancement of steady state levels at 18 hours of stimulation by OM and IL-1 alpha, or OM and PGE2. Probing for mRNA of the metalloproteinase inhibitor TIMP-1 showed that stimulation by OM, IL-1 alpha or PGE2 enhanced TIMP-1 levels. However, OM (alone) or PGE2 or both combined did not elevate the metalloproteinase stromelysin-1 mRNA signals. Analysis utilizing a rat IL-6 promoter-luciferase reporter gene construct showed that OM stimulation resulted in activation of transcription that synergistically enhanced IL-1-induced levels of reporter gene expression. These results show that although OM has minor effects on IL-6 production alone, the combination of OM and other mediators result in markedly enhanced IL-6 production by fibroblasts in vitro.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloendopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/OSM protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Oncostatin M,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Inhibitor of...
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1043-4666
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
40-7
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8003632-Animals,
pubmed-meshheading:8003632-Biological Assay,
pubmed-meshheading:8003632-Blotting, Northern,
pubmed-meshheading:8003632-Cell Line,
pubmed-meshheading:8003632-Cytokines,
pubmed-meshheading:8003632-Dinoprostone,
pubmed-meshheading:8003632-Dose-Response Relationship, Drug,
pubmed-meshheading:8003632-Drug Interactions,
pubmed-meshheading:8003632-Fibroblasts,
pubmed-meshheading:8003632-Gene Expression,
pubmed-meshheading:8003632-Glycoproteins,
pubmed-meshheading:8003632-Humans,
pubmed-meshheading:8003632-Interleukin-1,
pubmed-meshheading:8003632-Interleukin-6,
pubmed-meshheading:8003632-Kinetics,
pubmed-meshheading:8003632-Lung,
pubmed-meshheading:8003632-Matrix Metalloproteinase 3,
pubmed-meshheading:8003632-Metalloendopeptidases,
pubmed-meshheading:8003632-Oncostatin M,
pubmed-meshheading:8003632-Peptides,
pubmed-meshheading:8003632-Promoter Regions, Genetic,
pubmed-meshheading:8003632-RNA, Messenger,
pubmed-meshheading:8003632-Rats,
pubmed-meshheading:8003632-Recombinant Fusion Proteins,
pubmed-meshheading:8003632-Synovial Membrane,
pubmed-meshheading:8003632-Tissue Inhibitor of Metalloproteinases,
pubmed-meshheading:8003632-Transcription, Genetic,
pubmed-meshheading:8003632-Transfection
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pubmed:year |
1994
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pubmed:articleTitle |
Interaction between oncostatin M, interleukin 1 and prostaglandin E2 in induction of IL-6 expression in human fibroblasts.
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pubmed:affiliation |
Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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