Linked Life Data

8003511

Source:http://linkedlifedata.com/resource/pubmed/id/8003511

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1994-7-21
pubmed:abstractText
Parathyroid hormone-related protein (PTHrP) is expressed by malignant tumors and leads to the syndrome of humoral hypercalcemia of malignancy. It is also expressed by a wide variety of nonmalignant tissues, in which it appears to play distinct paracrine and/or autocrine roles. The human PTHrP gene encodes three cDNA-predicted initial translational products of 139, 141, and 173 amino acids. Most human cell lines contain mRNAs encoding all three PTHrP isoforms. The physiological rationale for the existence of these three highly similar transcripts is unknown. In order to determine whether the protein products derived from these three transcripts differ, we transfected Chinese hamster ovary (CHO) cells and rat insulinoma (RIN) cells individually with cDNAs encoding human PTHrP(1-139), PTHrP(1-141), and PTHrP(1-173). Cell extracts and conditioned medium were then chromatographed using reversed-phase HPLC and analyzed using region-specific PTHrP immunoassays. As we had previously observed in SKRC-1 (renal cell carcinoma) and RIN(1-141) cells, multiple amino-terminal PTHrP species as well as a separate midregion PTHrP species were identified in all six cell lines. In addition, both CHO and RIN cell lines transfected with the PTHrP(1-139) construct contained a previously unrecognized carboxy-terminal fragment that reacted with a PTHrP(109-138) antiserum. This carboxy-terminal fragment was physically distinct from the midregion fragment discovered earlier and was also present in conditioned medium, indicating that it is a secretory form, rather than a biosynthetic intermediate or a degradation product.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
7460-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8003511-Amino Acid Sequence, pubmed-meshheading:8003511-Animals, pubmed-meshheading:8003511-CHO Cells, pubmed-meshheading:8003511-Chromatography, Affinity, pubmed-meshheading:8003511-Chromatography, High Pressure Liquid, pubmed-meshheading:8003511-Cricetinae, pubmed-meshheading:8003511-Cricetulus, pubmed-meshheading:8003511-Culture Media, Conditioned, pubmed-meshheading:8003511-Humans, pubmed-meshheading:8003511-Immunohistochemistry, pubmed-meshheading:8003511-Molecular Sequence Data, pubmed-meshheading:8003511-Parathyroid Hormone, pubmed-meshheading:8003511-Parathyroid Hormone-Related Protein, pubmed-meshheading:8003511-Peptide Fragments, pubmed-meshheading:8003511-Protein Processing, Post-Translational, pubmed-meshheading:8003511-Proteins, pubmed-meshheading:8003511-Tumor Cells, Cultured
pubmed:year
1994
pubmed:articleTitle
Parathyroid hormone-related protein: evidence for isoform- and tissue-specific posttranslational processing.
pubmed:affiliation
Division of Endocrinology, West Haven Veterans Affairs Medical Center, Connecticut 06516.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't