Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-1-17
pubmed:abstractText
Human umbilical vein endothelial cells (HUVEC) were incubated for 24 hours with 0.1 mM or 0.3 mM of: [A] unmodified (U) Hb-FeIIO2; [B] UHb-FeIII; [C] UHb-FeIV-OH; [D] polymerized low molecular weight Hb (< 400 kDa); [E] polymerized high molecular weight Hb (< 1,020 kDa); [F] polymerized low molecular weight Hb + Endotoxin (2.5 EU/mL); [G] rTNF alpha 100 pg/mL; [H] rTNF alpha 400 pg/mL; [I] rTNF alpha 800 pg/mL. The medium of the incubation was tested for LDH (index of cell injury), and for cytokines GM-CSF and IL-1 alpha released by the cells. The data suggests that oxidation status of the iron in the Hb molecule and concentration of Hb play an important role in causing EC injury. The highest toxicity was observed when EC were incubated with 0.1 mM of UHb-FeIV-OH (ferryl-Hb) and no toxicity with 0.3 mM of Hb-FeIII (ferric-Hb). The direct stimulation of EC by Hb for the production of IL-1 was limited, related only to high molecular weight Hb polymers or to Hb+E, however GM-CSF expression was increased by almost all Hb forms. TNF induced dose-related injury (R2 = 0.986), and dose-related release of IL-1 (R2 = 0.977). A different EC reaction was observed on the release of GM-CSF. Intermediate levels of TNF (400 pg/mL) increased the expression of this cytokine, while high levels (800 pg/mL) blocked its release.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1073-1199
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
777-87
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:7994400-Animals, pubmed-meshheading:7994400-Blood Substitutes, pubmed-meshheading:7994400-Cattle, pubmed-meshheading:7994400-Cells, Cultured, pubmed-meshheading:7994400-Cross-Linking Reagents, pubmed-meshheading:7994400-Endothelium, Vascular, pubmed-meshheading:7994400-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:7994400-Hemoglobins, pubmed-meshheading:7994400-Humans, pubmed-meshheading:7994400-Interleukin-1, pubmed-meshheading:7994400-Iron, pubmed-meshheading:7994400-L-Lactate Dehydrogenase, pubmed-meshheading:7994400-Models, Biological, pubmed-meshheading:7994400-Molecular Weight, pubmed-meshheading:7994400-Oxidation-Reduction, pubmed-meshheading:7994400-Polymers, pubmed-meshheading:7994400-Solutions, pubmed-meshheading:7994400-Tumor Necrosis Factor-alpha
pubmed:year
1994
pubmed:articleTitle
Reaction of human endothelial cells to bovine hemoglobin solutions and tumor necrosis factor.
pubmed:affiliation
Texas Tech University Health Sciences Center, Department of Surgery, Lubbock 79430.
pubmed:publicationType
Journal Article