Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
50
pubmed:dateCreated
1995-1-12
pubmed:abstractText
We have demonstrated that the native proenzymatic form of Pseudomonas exotoxin A can be cleaved at its specific activation site by furin in intact Chinese hamster ovary cells or in vitro by furin in isolated membrane fractions from these cells. We have compared the activity of furin in cell membrane fractions with that of purified, recombinant human furin. We have verified that RPE.40, a Pseudomonas toxin-resistant mutant cell strain, is mutant in the fur gene, and we have demonstrated that these cells are deficient in cleavage of the toxin. We have also determined that this cleavage of Pseudomonas toxin by furin takes place at the authentic activation site to release the 37-kDa active fragment.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/ADP Ribose Transferases, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial, http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins, http://linkedlifedata.com/resource/pubmed/chemical/Exotoxins, http://linkedlifedata.com/resource/pubmed/chemical/Furin, http://linkedlifedata.com/resource/pubmed/chemical/Peptide Elongation Factor 2, http://linkedlifedata.com/resource/pubmed/chemical/Peptide Elongation Factors, http://linkedlifedata.com/resource/pubmed/chemical/Poly(ADP-ribose) Polymerases, http://linkedlifedata.com/resource/pubmed/chemical/Subtilisins, http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, http://linkedlifedata.com/resource/pubmed/chemical/anthrax toxin, http://linkedlifedata.com/resource/pubmed/chemical/toxA protein, Pseudomonas aeruginosa
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
269
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
31831-5
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7989356-ADP Ribose Transferases, pubmed-meshheading:7989356-Amino Acid Sequence, pubmed-meshheading:7989356-Animals, pubmed-meshheading:7989356-Antigens, Bacterial, pubmed-meshheading:7989356-Bacterial Toxins, pubmed-meshheading:7989356-CHO Cells, pubmed-meshheading:7989356-Cell Membrane, pubmed-meshheading:7989356-Cricetinae, pubmed-meshheading:7989356-Cytosol, pubmed-meshheading:7989356-Enzyme Activation, pubmed-meshheading:7989356-Exotoxins, pubmed-meshheading:7989356-Furin, pubmed-meshheading:7989356-Molecular Sequence Data, pubmed-meshheading:7989356-Peptide Elongation Factor 2, pubmed-meshheading:7989356-Peptide Elongation Factors, pubmed-meshheading:7989356-Poly(ADP-ribose) Polymerases, pubmed-meshheading:7989356-Subtilisins, pubmed-meshheading:7989356-Transfection, pubmed-meshheading:7989356-Virulence Factors
pubmed:year
1994
pubmed:articleTitle
Furin activates Pseudomonas exotoxin A by specific cleavage in vivo and in vitro.
pubmed:affiliation
Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, Burlington 05405.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't