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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0037119,
umls-concept:C0079784,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0205177,
umls-concept:C0205460,
umls-concept:C0323309,
umls-concept:C0376315,
umls-concept:C0935640,
umls-concept:C2911684
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pubmed:issue |
6
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pubmed:dateCreated |
1995-1-5
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pubmed:abstractText |
The human macrophage colony stimulating factor (hM-CSF) in its monomeric form has been over-produced in BmN cells and in silkworm larvae infected with the recombinant baculovirus Bm284M-CSF. The recombinant monomeric M-CSF (rhM-CSF) exhibited the activity of 8-14 x 10(4) units/ml of cell culture medium. When the insect larvae were infected with the recombinant virus, the maximum rhM-CSF was expressed 4-5 days post infection with an activity of 3 x 10(6) units/ml hemolymph. The monomeric rhM-CSF was purified to homogeneity through three steps of purification. A pilot purification yielded 1 mg of homogeneous monomeric rhM-CSF from 10 larvae. The purified rhM-CSF monomers gradually dimerized in vitro. In contrast, the crude or the semi-purified monomers did not dimerize in vitro, indicating that the presence of an unknown moiety in the rhM-CSF preparations obtained from hemolymph interfered with dimerization.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0177-3593
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
375
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
413-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7980874-Alkylation,
pubmed-meshheading:7980874-Animals,
pubmed-meshheading:7980874-Baculoviridae,
pubmed-meshheading:7980874-Blotting, Western,
pubmed-meshheading:7980874-Bombyx,
pubmed-meshheading:7980874-Bone Marrow,
pubmed-meshheading:7980874-Bone Marrow Cells,
pubmed-meshheading:7980874-Chromatography, Gel,
pubmed-meshheading:7980874-Chromatography, Ion Exchange,
pubmed-meshheading:7980874-DNA, Complementary,
pubmed-meshheading:7980874-Hemolymph,
pubmed-meshheading:7980874-Humans,
pubmed-meshheading:7980874-Larva,
pubmed-meshheading:7980874-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:7980874-Mice,
pubmed-meshheading:7980874-Mice, Inbred C57BL,
pubmed-meshheading:7980874-Receptor, Macrophage Colony-Stimulating Factor,
pubmed-meshheading:7980874-Recombinant Proteins,
pubmed-meshheading:7980874-Sulfhydryl Compounds
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pubmed:year |
1994
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pubmed:articleTitle |
Expression of biologically active monomeric form of human M-CSF in baculovirus infected silkworm, Bombyx mori.
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pubmed:affiliation |
Department of Biochemistry, Nanjing University, P.R. China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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