Linked Life Data

7975723

Source:http://linkedlifedata.com/resource/pubmed/id/7975723

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1994-12-8
pubmed:abstractText
1. A membrane-bound peroxidase activity was isolated and purified from pooled samples of human intrauterine tissue (embryo, placenta, amnion, chorion and uterine decidua) of 4-6 weeks of gestation. 2. The H2O2-dependent guaiacol oxidation catalysed by the purified peroxidase exhibited a specific activity of 10.82 +/- 1.81 mumol/min/mg protein. 3. The optimum conditions required for guaiacol oxidation catalysed by the peroxidase included 18 mM guaiacol, 400 microM H2O2, pH 9.0, and 2 micrograms/ml enzyme protein. The guaiacol oxidation was inhibited by KCN and NaN3 with IC50s of 17.6 and 22 microM, respectively. 4. Several other endogenous and exogenous chemicals also underwent H2O2-dependent oxidation catalysed by the purified peroxidase. These results suggest that peroxidase may be one of the major pathways of xenobiotic oxidation present in organogenesis-stage human conceptual tissues influencing the toxicity of chemicals to which the developing embryo is exposed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0049-8254
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
583-90
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Xenobiotic oxidation during early pregnancy in man: peroxidase catalysed chemical oxidation in conceptual tissues.
pubmed:affiliation
Toxicology Program, College of Public Health, MDC, University of South Florida, Tampa 33612.
pubmed:publicationType
Journal Article