Linked Life Data

7958809

Source:http://linkedlifedata.com/resource/pubmed/id/7958809

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1994-12-29
pubmed:abstractText
Using the method of P-element-mediated enhancer detection, 29 Drosophila melanogaster lines were obtained that carried P-1ArB vector insertions in chromosomes 2 and 3. The expression of the reporter gene lacZ at different developmental stages of the transformed lines was determined in color reactions for beta-galactosidase. Regions of vector integration were located using in situ hybridization. Subsequent electron-microscopic mapping of the transformed regions was performed using four lines (nos. 12, 41, 2, and 3). In the lines 12 and 41, lacZ was expressed in most tissues of embryos, larvae, and imagoes (including salivary glands), whereas in lines 2 and 3 its expression was observed only in embryos. Lines 12 and 2 showed the presence of insertions in the 85D9/10 and 86B4/6 interband regions, respectively. The absence of a novel band in line 3 could be associated with transposon integration into the band. In line 41, puffing of the transformed region was observed, which did not allow us to determine the presence of any novel structures in it. The novel structures in lines 12 and 2 looked like single bands with a similar DNA packing ratio of about 30. These bands were obviously polygenic, because the inserted vector contained four functionally different genes.
pubmed:language
rus
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0016-6758
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
927-33
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
[Cytogenetic analysis of insertions into drosophila interband polytene chromosomes].
pubmed:publicationType
Journal Article, English Abstract