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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1994-11-23
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pubmed:databankReference |
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pubmed:abstractText |
We report the identification and initial characterization of Drosophila melanogaster ankyrin. Oligonucleotide primers based on the spectrin-binding domain of human brain ankyrin were used to amplify Drosophila genomic DNA. A cloned 184-bp PCR product was used to isolate Drosophila ankyrin cDNAs. Ankyrin cDNA probes detected a 5.5-kb transcript from embryonic poly(A)+ RNA and a single polytene chromosome locus (101F-102A). The cDNA sequence encodes a 170-kDa protein that is 53% identical to human brain ankyrin (Ank2). Antibodies directed against a recombinant fragment of Drosophila ankyrin reacted with a 170-kDa polypeptide from Drosophila embryos, larvae, S2 cells, and adult flies. The ankyrin antibody coimmunoprecipitated alpha- and beta-spectrin with ankyrin in detergent extracts of Drosophila embryo membranes. Antibodies against Drosophila ankyrin, alpha-spectrin and beta-spectrin were used to detect these proteins in wild-type and alpha-spectrin-mutant larvae. alpha-Spectrin levels were greatly diminished in mutant larvae, but levels of ankyrin and beta-spectrin were indistinguishable from wild type. The persistence of ankyrin and beta-spectrin may explain the relatively mild phenotype of alpha-spectrin mutants during early Drosophila development.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-1340461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-1423619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-1476738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-1533619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-1592233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-1631106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-1651488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-1830053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-2137557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-2164888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-2255917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-2271059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-2452986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-2675425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-2805067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-2808524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-2824526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-3023391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-3039371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-3047011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-3199441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-3680372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-3822832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-3935325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-6234993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-6546423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-8162854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-8227202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-8276898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-8280463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-8458865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937942-8471772
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10285-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7937942-Amino Acid Sequence,
pubmed-meshheading:7937942-Animals,
pubmed-meshheading:7937942-Ankyrins,
pubmed-meshheading:7937942-Antibodies,
pubmed-meshheading:7937942-Base Sequence,
pubmed-meshheading:7937942-Blotting, Southern,
pubmed-meshheading:7937942-Brain,
pubmed-meshheading:7937942-DNA, Complementary,
pubmed-meshheading:7937942-DNA Primers,
pubmed-meshheading:7937942-DNA Probes,
pubmed-meshheading:7937942-Drosophila melanogaster,
pubmed-meshheading:7937942-Humans,
pubmed-meshheading:7937942-Immunoblotting,
pubmed-meshheading:7937942-Larva,
pubmed-meshheading:7937942-Molecular Sequence Data,
pubmed-meshheading:7937942-Polymerase Chain Reaction,
pubmed-meshheading:7937942-Recombinant Proteins,
pubmed-meshheading:7937942-Restriction Mapping,
pubmed-meshheading:7937942-Spectrin
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pubmed:year |
1994
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pubmed:articleTitle |
Ankyrin and beta-spectrin accumulate independently of alpha-spectrin in Drosophila.
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pubmed:affiliation |
Department of Pharmacological and Physiological Sciences, University of Chicago, IL 60637.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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