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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1994-7-7
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pubmed:abstractText |
We have used antisense phosphorothioate oligonucleotides to define the role played by proliferating cell nuclear antigen (PCNA) in neointimal accumulation of smooth muscle cells in a rat carotid artery injury model. The short-term extraluminal delivery of 250 nmol of antisense oligonucleotides, but not control oligonucleotides, immediately after arterial injury produces a 77% suppression of PCNA mRNA after 24 h and a 52% decrease in the frequency of medial smooth muscle cells expressing PCNA after 72 h. This reduction in PCNA expression is accompanied by a 59% decrease in the frequency of proliferating medial smooth muscle cells at 3 d as measured by BudR staining and an 80% decrease in neointimal accumulation assessed morphometrically at 2 wk. Thus, the expression of PCNA is required for medial smooth muscle cell growth in vivo and for neointimal formation after arterial injury.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-102692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-1353419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-1371124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-1380154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-1522889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-1537845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-1551207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-1653454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-1708128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-1712444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-1991900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-2023924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-2060114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-2154504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-2461588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-2665077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-2674692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-2869964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-2882423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-2894900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-2897717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-2927077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-3276731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-3285807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-3516443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-3546073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-8104336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-8381210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-8416967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-8421701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7911126-8486791
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2351-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7911126-Animals,
pubmed-meshheading:7911126-Base Sequence,
pubmed-meshheading:7911126-Carotid Arteries,
pubmed-meshheading:7911126-Cell Division,
pubmed-meshheading:7911126-Cell Line,
pubmed-meshheading:7911126-Hyperplasia,
pubmed-meshheading:7911126-Molecular Sequence Data,
pubmed-meshheading:7911126-Muscle, Smooth, Vascular,
pubmed-meshheading:7911126-Nuclear Proteins,
pubmed-meshheading:7911126-Oligonucleotides, Antisense,
pubmed-meshheading:7911126-Proliferating Cell Nuclear Antigen,
pubmed-meshheading:7911126-Rats,
pubmed-meshheading:7911126-Rats, Sprague-Dawley
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pubmed:year |
1994
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pubmed:articleTitle |
Antisense proliferating cell nuclear antigen oligonucleotides inhibit intimal hyperplasia in a rat carotid artery injury model.
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pubmed:affiliation |
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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