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rdf:type |
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lifeskim:mentions |
umls-concept:C0008109,
umls-concept:C0021764,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0079633,
umls-concept:C0242299,
umls-concept:C0330390,
umls-concept:C0567416,
umls-concept:C0597357,
umls-concept:C0936012,
umls-concept:C1145667
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pubmed:issue |
10
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pubmed:dateCreated |
1995-4-14
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pubmed:databankReference |
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pubmed:abstractText |
We have demonstrated that the orphan receptor representing the putative mouse (mu) homolog of the human (hu) interleukin-8 receptor beta (IL-8R beta) binds the mouse N51 cytokine, also known as KC. The muIL-8R beta gene was constitutively expressed in NIH 3T3 cells (NIH-muIL-8R beta). Cells and plasma membranes from the NIH-muIL-8R beta clone showed binding of 125I-N51 that was displaced by unlabeled N51. Other related cytokines were assayed for their ability to displace 125I-N51. MIP-2 and GRO alpha/MGSA competed as well as N51 for the receptor, but huIL-8 and NAP-2 did not compete at all. Chimeric molecules between IL-8 and N51 were used to extend the binding analysis. The segment between the conserved cysteines 2 and 3, named domain I; cysteines 3 and 4, domain II; and cysteine 4 and the C terminus, domain III of IL-8 were replaced by the corresponding domains of N51 and vice versa. When studying the binding of 125I-N51 and the hybrid molecules to the receptor, we observed that chimeras of N51 containing either domain I, II, or III of IL-8 were agonists of N51, and chimeras of IL-8 containing domain II or III of N51 were partial agonists of N51. These results demonstrate that domain I of N51 does not confer binding specificity and suggest that the region from the third cysteine to the C terminus of the N51 molecule is more important for binding to muIL-8R beta.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CXCL1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL1,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcl1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Disulfides,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-8A,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-8B,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4987-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:7890604-3T3 Cells,
pubmed-meshheading:7890604-Amino Acid Sequence,
pubmed-meshheading:7890604-Animals,
pubmed-meshheading:7890604-Base Sequence,
pubmed-meshheading:7890604-Binding, Competitive,
pubmed-meshheading:7890604-Binding Sites,
pubmed-meshheading:7890604-Chemokine CXCL1,
pubmed-meshheading:7890604-Chemokines, CXC,
pubmed-meshheading:7890604-Chemotactic Factors,
pubmed-meshheading:7890604-Cloning, Molecular,
pubmed-meshheading:7890604-Conserved Sequence,
pubmed-meshheading:7890604-Cysteine,
pubmed-meshheading:7890604-DNA Primers,
pubmed-meshheading:7890604-Disulfides,
pubmed-meshheading:7890604-Growth Substances,
pubmed-meshheading:7890604-Humans,
pubmed-meshheading:7890604-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:7890604-Iodine Radioisotopes,
pubmed-meshheading:7890604-Kinetics,
pubmed-meshheading:7890604-Mice,
pubmed-meshheading:7890604-Molecular Sequence Data,
pubmed-meshheading:7890604-Neoplasm Proteins,
pubmed-meshheading:7890604-Polymerase Chain Reaction,
pubmed-meshheading:7890604-Receptors, Interleukin,
pubmed-meshheading:7890604-Receptors, Interleukin-8A,
pubmed-meshheading:7890604-Receptors, Interleukin-8B,
pubmed-meshheading:7890604-Recombinant Proteins,
pubmed-meshheading:7890604-Sequence Homology, Amino Acid,
pubmed-meshheading:7890604-Transfection
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pubmed:year |
1995
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pubmed:articleTitle |
The orphan mouse receptor interleukin (IL)-8R beta binds N51. Structure-function analysis using N51/IL-8 chimeric molecules.
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pubmed:affiliation |
Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543-4000.
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pubmed:publicationType |
Journal Article,
Comparative Study
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