Linked Life Data

7881460

Source:http://linkedlifedata.com/resource/pubmed/id/7881460

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-4-13
pubmed:abstractText
In the fowl the primordial germ cells accumulate in the germinal crescent to the anterior of the two-day embryo. A simple ballistic device has been used to fire tungsten particles (mean diameter 1.5 microns) into this region. By coating these projectiles with vector DNA it is possible to transfect these cells. Hatchlings produced by this technique were raised to sexual maturity and shown to contain the foreign DNA in their sperm. G1 offspring containing this DNA were also produced in roughly 20% of these cockerels. In the majority of cases the vector DNA disappeared from the G1 generation as they matured suggesting that in these cases it had been transmitted episomally.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0962-8819
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:geneSymbol
neo
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
26-9
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Ballistic transfection of avian primordial germ cell in ovo.
pubmed:affiliation
School of Animal and Microbial Sciences, University of Reading, UK.
pubmed:publicationType
Journal Article