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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2 Pt 2
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pubmed:dateCreated |
1995-3-20
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pubmed:abstractText |
The initial segment of the inner medullary collecting duct (IMCDi) absorbs Na+ by an electrogenic mechanism and plays an important role in regulating the composition and volume of the urine. The purpose of the present study was to establish a permanent cell line derived from the IMCDi, which has the ion transport properties of the IMCDi in vivo. To this end, we isolated IMCD cells from the IMCDi of a mouse, Tg(SV40E) Bri 7, transgenic for the early region of SV40 (large T antigen) and established a permanent cell line, mIMCD-K2, by clonal dilution. mIMCD-K2 cells retain many differentiated characteristics of the IMCDi, including amiloride-sensitive electrogenic Na+ absorption stimulated by nanomolar concentrations of aldosterone. Aldosterone (1.5 x 10(-6) M) increased Na+ absorption from 0.2 +/- 0.1 to 4.6 +/- 1.7 microA/cm2. In addition, the cells secrete Cl- by an electrogenic mechanism at a rate of 0.5 +/- 0.1 microA/cm2. We propose that IMCDi cells either absorb or secrete NaCl depending on NaCl homeostasis. The mIMCD-K2 cell line should be useful for studying the cellular mechanisms responsible for electrogenic Na+ and Cl- transport in the IMCDi.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aldosterone,
http://linkedlifedata.com/resource/pubmed/chemical/Amiloride,
http://linkedlifedata.com/resource/pubmed/chemical/Anthranilic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Potassium-Exchanging ATPase,
http://linkedlifedata.com/resource/pubmed/chemical/fenamic acid
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0002-9513
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
268
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
F347-55
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:7864176-Absorption,
pubmed-meshheading:7864176-Aldosterone,
pubmed-meshheading:7864176-Amiloride,
pubmed-meshheading:7864176-Animals,
pubmed-meshheading:7864176-Anthranilic Acids,
pubmed-meshheading:7864176-Biological Transport,
pubmed-meshheading:7864176-Cell Division,
pubmed-meshheading:7864176-Cell Line,
pubmed-meshheading:7864176-Chlorides,
pubmed-meshheading:7864176-Electrophysiology,
pubmed-meshheading:7864176-Kidney Medulla,
pubmed-meshheading:7864176-Kidney Tubules, Collecting,
pubmed-meshheading:7864176-Male,
pubmed-meshheading:7864176-Mice,
pubmed-meshheading:7864176-Mice, Transgenic,
pubmed-meshheading:7864176-Sodium,
pubmed-meshheading:7864176-Sodium-Potassium-Exchanging ATPase
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pubmed:year |
1995
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pubmed:articleTitle |
Electrogenic sodium absorption and chloride secretion by an inner medullary collecting duct cell line (mIMCD-K2).
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pubmed:affiliation |
Department of Physiology, Dartmouth Medical School, Hanover, New Hampshire 03755.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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