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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1995-3-1
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pubmed:abstractText |
The murine macrophage inflammatory protein 1 beta mRNA (MIP-1 beta) is rapidly and transiently induced in macrophages by lipopolysaccharide (LPS), serum or cycloheximide. Functional studies of the MIP-1 beta proximal promoter indicate that it is cell-specific, and serum- and LPS-responsive in macrophages. A 76-bp proximal promoter sequence (-51 to -127 bp) confers cell-specific and LPS-inducible activity when placed upstream from a heterologous promoter in both orientations. One essential cis-regulatory element within the enhancer-like sequence is an activating transcription factor/cAMP response element (CRE)-binding protein (ATF/CREB)-binding site, although the promoter is not cAMP responsive. Electrophoretic mobility shift assays and mutational analyses suggest that the promoter site is bound by nuclear protein complexes containing cAMP-independent members of the ATF/CREB family of proteins and c-Jun, and are functionally distinct from the AP1-related TPA-response element (TRE) binding activity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Activating Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL4,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Inflammatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monokines,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
152
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pubmed:geneSymbol |
MIP-1&bgr;,
bFP6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
173-9
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pubmed:dateRevised |
2010-2-4
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pubmed:meshHeading |
pubmed-meshheading:7835696-Activating Transcription Factors,
pubmed-meshheading:7835696-Animals,
pubmed-meshheading:7835696-Base Sequence,
pubmed-meshheading:7835696-Binding Sites,
pubmed-meshheading:7835696-Blood Proteins,
pubmed-meshheading:7835696-Cell Line,
pubmed-meshheading:7835696-Chemokine CCL4,
pubmed-meshheading:7835696-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:7835696-Cytokines,
pubmed-meshheading:7835696-Macrophage Inflammatory Proteins,
pubmed-meshheading:7835696-Mice,
pubmed-meshheading:7835696-Molecular Sequence Data,
pubmed-meshheading:7835696-Monokines,
pubmed-meshheading:7835696-Mutagenesis, Site-Directed,
pubmed-meshheading:7835696-Oligodeoxyribonucleotides,
pubmed-meshheading:7835696-Promoter Regions, Genetic,
pubmed-meshheading:7835696-RNA, Messenger,
pubmed-meshheading:7835696-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:7835696-Transcription, Genetic,
pubmed-meshheading:7835696-Transcription Factor AP-1,
pubmed-meshheading:7835696-Transcription Factors
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pubmed:year |
1995
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pubmed:articleTitle |
An ATF/CREB-binding site is essential for cell-specific and inducible transcription of the murine MIP-1 beta cytokine gene.
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pubmed:affiliation |
ICRF Cancer Medicine Research Unit, St. James's University Hospital, Leeds, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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