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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1995-2-24
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pubmed:abstractText |
Sorcin, a 22 kDa calcium binding protein present in abundance in cardiac tissue and in multi-drug resistant cells and previously described as a soluble protein, is now shown to undergo a calcium-dependent translocation process from the cytosol to cellular membranes in both systems. The translocation process takes place also in E. coli BL21 cells that express recombinant sorcin, r-sorcin, and can be exploited in the purification of the protein. Calcium binding to purified r-sorcin occurs at micromolar concentrations of the metal and is accompanied by a conformational change that renders the protein soluble in the non-ionic detergent Triton X-114. This finding suggests that lipids are the target of sorcin on cellular membranes. The possible significance of the calcium-dependent translocation of sorcin in the specialized functions of sorcin-expressing cells is discussed.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
357
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
230-4
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7835417-Animals,
pubmed-meshheading:7835417-Base Sequence,
pubmed-meshheading:7835417-Biological Transport,
pubmed-meshheading:7835417-Calcium,
pubmed-meshheading:7835417-Calcium-Binding Proteins,
pubmed-meshheading:7835417-Cell Membrane,
pubmed-meshheading:7835417-DNA Primers,
pubmed-meshheading:7835417-Detergents,
pubmed-meshheading:7835417-Drug Resistance, Multiple,
pubmed-meshheading:7835417-Escherichia coli,
pubmed-meshheading:7835417-Molecular Sequence Data,
pubmed-meshheading:7835417-Myocardium,
pubmed-meshheading:7835417-Polyethylene Glycols,
pubmed-meshheading:7835417-Protein Conformation,
pubmed-meshheading:7835417-Rabbits
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pubmed:year |
1995
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pubmed:articleTitle |
Calcium-dependent translocation of sorcin to membranes: functional relevance in contractile tissue.
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pubmed:affiliation |
Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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