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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-2-7
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pubmed:abstractText |
IL-4 and IFN-gamma positively and negatively regulate allergic inflammation. To determine the regulatory mechanisms of eosinopoiesis by cytokines, we examined the effect of recombinant IL-4 and IFN-gamma and of anti-IL-4 and anti-IFN-gamma antibodies on IL-3- and IL-5-induced eosinophil differentiation from human umbilical cord blood mononuclear cells. rhIL-4 (10-300 U/ml) inhibited IL-3- and IL-5-induced eosinophil differentiation from cord blood mononuclear cells on day 28 of culture by 62-81% in a concentration-dependent manner. rhIFN-gamma (5-500 U/ml) also inhibited IL-3- and IL-5-induced eosinophil differentiation by 80-99% in a concentration-dependent manner. The inhibitory effect of rhIL-4 and rhIFN-gamma was observed only when rhIL-4 or rhIFN-gamma were present in the culture from day 0 to day 14, but not from day 15 to day 28. Addition of anti-IL-4 antibody to the culture enhanced IL-3- and IL-5-induced eosinophil differentiation on day 28 of culture by 30%, whereas anti-IL-2 MoAb and anti-IFN-gamma MoAb had no significant effect. These results indicate that IL-4 and IFN-gamma have inhibitory effects on IL-3- and IL-5-induced eosinophil differentiation from its progenitor cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-1379084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-1698867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-1940348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-1987770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-2411798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-2664346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-2823259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-2971726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-3100961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-3258425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-3484427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-3484428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-3495002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-3496134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-3498940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-3498944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-3919982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-603764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-6159105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-6193183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7813104-7904900
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
124-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7813104-Antibodies,
pubmed-meshheading:7813104-Cell Differentiation,
pubmed-meshheading:7813104-Cells, Cultured,
pubmed-meshheading:7813104-Eosinophils,
pubmed-meshheading:7813104-Humans,
pubmed-meshheading:7813104-Interferon-gamma,
pubmed-meshheading:7813104-Interleukin-3,
pubmed-meshheading:7813104-Interleukin-4,
pubmed-meshheading:7813104-Interleukin-5,
pubmed-meshheading:7813104-Leukocytes,
pubmed-meshheading:7813104-Stem Cells
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pubmed:year |
1995
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pubmed:articleTitle |
Effect of IL-4 and interferon-gamma (IFN-gamma) on IL-3- and IL-5-induced eosinophil differentiation from human cord blood mononuclear cells.
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pubmed:affiliation |
Department of Internal Medicine, Chiba University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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