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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-1-24
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pubmed:abstractText |
The MIC of isoniazid, peroxidase-catalase expression, and the presence of the katG gene for 102 Mycobacterium tuberculosis isolates from patients in São Paulo were compared. Fifty-three isoniazid-resistant and 49 isoniazid-sensitive isolates were analyzed by polymerase chain reaction (PCR) for the presence of katG sequences. All isoniazid-sensitive and 43 (81%) isoniazid-resistant isolates expressed catalase (P = .001). None of isoniazid-sensitive and 4 (7%) of 53 isoniazid-resistant isolates lacked katG sequences. Among 6 isolates with MICs > 50 micrograms/mL, 5 (83%) did not express catalase and 2 lacked katG sequences; only 1 had complete gene deletion shown by Southern blot analysis. These findings indicate a correlation between loss of catalase and isoniazid resistance among highly resistant isolates, but these isolates were a small proportion of resistant clinical M. tuberculosis isolates from São Paulo.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1899
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
171
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pubmed:geneSymbol |
katG
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
237-40
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7798672-Bacterial Proteins,
pubmed-meshheading:7798672-Base Sequence,
pubmed-meshheading:7798672-Blotting, Southern,
pubmed-meshheading:7798672-Brazil,
pubmed-meshheading:7798672-Catalase,
pubmed-meshheading:7798672-DNA Primers,
pubmed-meshheading:7798672-Drug Resistance, Microbial,
pubmed-meshheading:7798672-Genes, Bacterial,
pubmed-meshheading:7798672-Humans,
pubmed-meshheading:7798672-Isoniazid,
pubmed-meshheading:7798672-Molecular Sequence Data,
pubmed-meshheading:7798672-Mycobacterium tuberculosis,
pubmed-meshheading:7798672-Peroxidases,
pubmed-meshheading:7798672-Polymerase Chain Reaction,
pubmed-meshheading:7798672-Tuberculosis, Pulmonary
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pubmed:year |
1995
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pubmed:articleTitle |
Catalase expression, katG, and MIC of isoniazid for Mycobacterium tuberculosis isolates from São Paulo, Brazil.
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pubmed:affiliation |
Setor de Micobacterias, Instituto Adolfo Lutz, São Paulo, Brazil.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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