Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2-3
|
| pubmed:dateCreated |
1995-6-20
|
| pubmed:abstractText |
We previously demonstrated stable integration of a transduced thymidine kinase (TK)-neo gene into immature and replatable stem and progenitor cells, as assessed by the presence of the gene in second-generation colonies. To evaluate whether this integration was still present in third- and fourth-generation colonies, nonadherent low-density T-lymphocyte-depleted (NALT-) cells from human umbilical cord blood were prestimulated with recombinant human (rhu) erythropoietin (Epo), steel factor (SLF), interleukin-3 (IL-3), granulocyte-macrophage (GM) colony-stimulating factor (CSF), and granulocyte (G)-CSF. Prestimulated NALT- cells were incubated with retroviral-containing supernatant obtained from TK-neo vector-producing cells, washed, and assayed for colony formation in the presence of Epo, SLF, IL-3, GM-CSF, and G-CSF -/+ G418. The results confirmed that the TK-neo gene could be efficiently introduced into hematopoietic progenitor cells without stromal cells as a source of virus. As previously reported, proviral integration was detected in primary G418R-colonies, and in second-generation replated colonies derived from G418R granulocyte erythroid macrophage megakaryocyte colony-forming units and high-proliferative potential colony-forming cells (HPP-CFCs). Moreover, we now document that proviral integration was apparent in cells from colonies derived from third- and fourth-generation replated HPP-CFC, suggesting a high degree of stable integration of the transduced gene.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Recombinant,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Hematopoietic Cell Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Kanamycin Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Neomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotransferases (Alcohol Group...,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine Kinase
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0340-4684
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
20
|
| pubmed:geneSymbol |
neo
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
525-30
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7749119-Cells, Cultured,
pubmed-meshheading:7749119-Colony-Forming Units Assay,
pubmed-meshheading:7749119-Connective Tissue,
pubmed-meshheading:7749119-Culture Techniques,
pubmed-meshheading:7749119-DNA, Recombinant,
pubmed-meshheading:7749119-DNA, Viral,
pubmed-meshheading:7749119-Drug Resistance,
pubmed-meshheading:7749119-Fetal Blood,
pubmed-meshheading:7749119-Genes, Synthetic,
pubmed-meshheading:7749119-Genetic Vectors,
pubmed-meshheading:7749119-Hematopoietic Cell Growth Factors,
pubmed-meshheading:7749119-Hematopoietic Stem Cells,
pubmed-meshheading:7749119-Humans,
pubmed-meshheading:7749119-Kanamycin Kinase,
pubmed-meshheading:7749119-Neomycin,
pubmed-meshheading:7749119-Phosphotransferases (Alcohol Group Acceptor),
pubmed-meshheading:7749119-Proviruses,
pubmed-meshheading:7749119-Recombinant Fusion Proteins,
pubmed-meshheading:7749119-Retroviridae,
pubmed-meshheading:7749119-Thymidine Kinase,
pubmed-meshheading:7749119-Virus Integration
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Stable integration of retrovirally transduced genes into human umbilical cord blood high-proliferative potential colony-forming cells (HPP-CFC) as assessed after multiple HPP-CFC colony replatings in vitro.
|
| pubmed:affiliation |
Department of Medicine (Hematology/Oncology), University School of Medicine, Indianapolis, Indiana 46202-5121, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|