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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1995-6-15
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pubmed:abstractText |
Addition of sphingosine 1-phosphate induces proliferation of quiescent Swiss 3T3 fibroblasts by unknown mechanisms. To identify the pathways involved, the ability of sphingosine 1-phosphate to activate mitogen-activated protein (MAP) kinase was studied. Sphingosine 1-phosphate rapidly activated the Raf/MAP kinase kinase (MKK)/MAP kinase pathway, and the concentration dependence for MAP kinase activation correlated with that for induction of DNA synthesis. Both MKK1 and MKK2 were activated by sphingosine 1-phosphate, assessed by specific immune complex kinase assays. Prior treatment of the Swiss 3T3 cells with pertussis toxin inhibited 70-80% of the sphingosine 1-phosphate-stimulated MAP kinase activity. Thus, one of the direct or indirect targets of exogenous sphingosine 1-phosphate appears to be a G(i)/G(o) protein.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lysophospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-raf,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sphingosine,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella,
http://linkedlifedata.com/resource/pubmed/chemical/platelet-derived growth factor BB,
http://linkedlifedata.com/resource/pubmed/chemical/sphingosine 1-phosphate
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11484-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7744787-3T3 Cells,
pubmed-meshheading:7744787-Animals,
pubmed-meshheading:7744787-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:7744787-Cell Division,
pubmed-meshheading:7744787-DNA,
pubmed-meshheading:7744787-DNA Replication,
pubmed-meshheading:7744787-Dose-Response Relationship, Drug,
pubmed-meshheading:7744787-Enzyme Activation,
pubmed-meshheading:7744787-Fibroblasts,
pubmed-meshheading:7744787-GTP-Binding Proteins,
pubmed-meshheading:7744787-Kinetics,
pubmed-meshheading:7744787-Lysophospholipids,
pubmed-meshheading:7744787-Mice,
pubmed-meshheading:7744787-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:7744787-Pertussis Toxin,
pubmed-meshheading:7744787-Platelet-Derived Growth Factor,
pubmed-meshheading:7744787-Protein Kinases,
pubmed-meshheading:7744787-Protein-Serine-Threonine Kinases,
pubmed-meshheading:7744787-Proto-Oncogene Proteins,
pubmed-meshheading:7744787-Proto-Oncogene Proteins c-raf,
pubmed-meshheading:7744787-Recombinant Proteins,
pubmed-meshheading:7744787-Sphingosine,
pubmed-meshheading:7744787-Virulence Factors, Bordetella
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pubmed:year |
1995
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pubmed:articleTitle |
Sphingosine 1-phosphate rapidly activates the mitogen-activated protein kinase pathway by a G protein-dependent mechanism.
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pubmed:affiliation |
Howard Hughes Medical Institute, Department of Medicine, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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