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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1995-5-30
|
| pubmed:abstractText |
The generation of contiguous physical maps is often complicated by a variety of factors including the type of cloning system used. Here we describe procedures for the isolation, rapid characterization, and physical mapping of large-insert recombinant bacterial clones from total human genomic BAC (bacterial artificial chromosome) and PAC (P1-derived artificial chromosome) libraries containing clones with an average insert size of 150 kbp. After initial isolation, the clones were subjected to a variety of fingerprinting procedures including inter-Alu PCR, semiautomated fluorescent finger-printing, and EcoRI restriction fragment mapping. Individual BAC and PAC clones were also used as probes to interrogate arrayed chromosome 19-specific cosmid libraries. The combination of analyses facilitated the identification of chromosome-specific large-insert clones as well as the construction of a large (1.2 Mb) high-resolution BAC, PAC, and cosmid contig in 19q13.2, spanning the region from the carcinoembryonic antigen gene family to the X-ray repair cross complementing 1 DNA repair gene. This type of approach directly demonstrates the utility of large-insert recombinant bacterial clones for the construction of contiguous physical maps of entire chromosomes.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0003-2697
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
20
|
| pubmed:volume |
224
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
564-71
|
| pubmed:dateRevised |
2006-4-13
|
| pubmed:meshHeading |
pubmed-meshheading:7733459-Base Sequence,
pubmed-meshheading:7733459-Blotting, Southern,
pubmed-meshheading:7733459-Chromosome Mapping,
pubmed-meshheading:7733459-Chromosomes, Bacterial,
pubmed-meshheading:7733459-Chromosomes, Human, Pair 19,
pubmed-meshheading:7733459-Cloning, Molecular,
pubmed-meshheading:7733459-Cosmids,
pubmed-meshheading:7733459-DNA Fingerprinting,
pubmed-meshheading:7733459-Fluorescence,
pubmed-meshheading:7733459-Genome, Human,
pubmed-meshheading:7733459-Humans,
pubmed-meshheading:7733459-Molecular Sequence Data,
pubmed-meshheading:7733459-Polymerase Chain Reaction,
pubmed-meshheading:7733459-Restriction Mapping
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Assembly of high-resolution bacterial artificial chromosome, P1-derived artificial chromosome, and cosmid contigs.
|
| pubmed:affiliation |
Human Genome Center, Lawrence Livermore National Laboratory, Livermore, California 94551, USA.
|
| pubmed:publicationType |
Journal Article
|