Linked Life Data

7729689

Source:http://linkedlifedata.com/resource/pubmed/id/7729689

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1995-6-1
pubmed:abstractText
mef2 encodes the only apparent Drosophila homolog of the vertebrate myocyte-specific enhancer factor 2 (MEF2). We show herein that the Drosophila MEF2 protein is expressed throughout the mesoderm following gastrulation. Later in embryogenesis, its expression is maintained in precursors and differentiated cells of the somatic and visceral musculature, as well as the heart. We have characterized genetic deficiencies and EMS-induced point mutations that result in complete loss of MEF2 protein in homozygous mutant embryos. These embryos exhibit a dramatic absence of myosin heavy chain (MHC)-expressing myoblasts and lack differentiated muscle fibers. Examination of earlier events of muscle development indicates that the specification and early differentiation of somatic muscle precursors are not affected because even-skipped-, nautilus-, and beta 3-tubulin-expressing myoblasts are present. However, these partially differentiated cells are unable to undergo further differentiation to form muscle fibers in the absence of mef2. The later aspects of differentiation of the visceral mesoderm and the heart are also disrupted in mef2 mutant embryos, although the specification and early development of these tissues appear unaffected. Midgut morphogenesis is disrupted in the mutant embryos, presumably as a consequence of abnormal development of the visceral mesoderm. In the heart, the cardial cells do not express MHC. These results indicate that MEF2 is required for later aspects of differentiation of the three major types of musculature, which include body wall muscles, gut musculature, and the heart, in the Drosophila embryo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0890-9369
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
730-41
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7729689-Amino Acid Sequence, pubmed-meshheading:7729689-Animals, pubmed-meshheading:7729689-Base Sequence, pubmed-meshheading:7729689-Cell Differentiation, pubmed-meshheading:7729689-DNA-Binding Proteins, pubmed-meshheading:7729689-Drosophila, pubmed-meshheading:7729689-Drosophila Proteins, pubmed-meshheading:7729689-Genetic Complementation Test, pubmed-meshheading:7729689-Heart, pubmed-meshheading:7729689-Histocompatibility Antigens, pubmed-meshheading:7729689-Mesoderm, pubmed-meshheading:7729689-Molecular Sequence Data, pubmed-meshheading:7729689-Muscle, Skeletal, pubmed-meshheading:7729689-Muscle, Smooth, pubmed-meshheading:7729689-Muscles, pubmed-meshheading:7729689-Mutagenesis, pubmed-meshheading:7729689-Myogenic Regulatory Factors, pubmed-meshheading:7729689-Myosins, pubmed-meshheading:7729689-Point Mutation, pubmed-meshheading:7729689-Stem Cells, pubmed-meshheading:7729689-Transcription Factors, pubmed-meshheading:7729689-Viscera
pubmed:year
1995
pubmed:articleTitle
Drosophila MEF2, a transcription factor that is essential for myogenesis.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't