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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1995-5-10
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pubmed:abstractText |
To identify proteins involved in the formation of replication complexes at the 3' end of poliovirus negative-strand RNA, a combined in vitro biochemical and in vivo genetic approach was used. Five subgenomic cDNA constructs were generated to transcribe different negative-strand RNA fragments. In UV cross-linking assays, distinct differences in binding of proteins in extracts from poliovirus-infected and uninfected cells to virus-specific, radiolabeled transcripts were observed. Two proteins present in extracts from poliovirus-infected cells with approximate molecular masses of 36 and 38 kDa were shown to cross-link to the 3' end of poliovirus negative-strand RNA. Appearance of the 36- and 38-kDa proteins in UV cross-linking assays can be detected 3 to 3.5 h after infection, and cross-linking reaches maximum levels by 5 h after infection. The binding site for the 36-kDa protein overlaps with the computer-predicted loop b region of stem-loop I, the so-called cloverleaf structure, and the RNA sequence of this region is required for efficient binding. Transfection of full-length, positive-sense RNA containing a five-nucleotide substitution (positions 20 to 25) in the loop b region of stem-loop I into tissue culture cells yielded only viral isolates with a reversion at position 24 (U-->C). This finding demonstrates that the wild-type cytidine residue at position 24 is essential for virus replication. RNA binding studies with transcripts corresponding to the 3' end of negative-strand RNA suggest that complex formation with the 36-kDa protein plays an essential role during the viral life cycle.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-103625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-1315956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-1321289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-1355457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-1602550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-1652694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-1661029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-1731089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-1846205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-1850039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-198796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-2165605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-2169386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-2170027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-2535728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-2547075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-2548189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-2835660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-2836612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-2838971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-2839775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-3001703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-3003384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-3010307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-3018300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-4287584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-6163133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-6244109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-6252335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-6280378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-6288718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-6324172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-7929441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-8039507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-8207812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-8230443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-8230467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-8253083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-8380467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7707521-8500177
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2954-61
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7707521-Base Sequence,
pubmed-meshheading:7707521-Binding Sites,
pubmed-meshheading:7707521-Cross-Linking Reagents,
pubmed-meshheading:7707521-DNA, Complementary,
pubmed-meshheading:7707521-HeLa Cells,
pubmed-meshheading:7707521-Humans,
pubmed-meshheading:7707521-Molecular Sequence Data,
pubmed-meshheading:7707521-Mutation,
pubmed-meshheading:7707521-Nucleic Acid Conformation,
pubmed-meshheading:7707521-Poliovirus,
pubmed-meshheading:7707521-RNA, Viral,
pubmed-meshheading:7707521-Ribonucleoproteins,
pubmed-meshheading:7707521-Ultraviolet Rays,
pubmed-meshheading:7707521-Virus Replication
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pubmed:year |
1995
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pubmed:articleTitle |
Poliovirus infection enhances the formation of two ribonucleoprotein complexes at the 3' end of viral negative-strand RNA.
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pubmed:affiliation |
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92717, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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