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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0001613,
umls-concept:C0007776,
umls-concept:C0017262,
umls-concept:C0021753,
umls-concept:C0022116,
umls-concept:C0022655,
umls-concept:C0035696,
umls-concept:C0185117,
umls-concept:C0205234,
umls-concept:C0205374,
umls-concept:C0521115,
umls-concept:C0599295,
umls-concept:C1456820,
umls-concept:C1705964,
umls-concept:C1719822,
umls-concept:C2911684
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pubmed:issue |
2-3
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pubmed:dateCreated |
1995-5-8
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pubmed:abstractText |
The expression of tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) mRNAs was significantly increased in the rat ischemic cortex following temporary occlusion of the middle cerebral artery (TMCAO) with reperfusion. Northern blot analysis demonstrated that the induction of TNF-alpha and IL-1 beta mRNAs occurred as early as 1 h after reperfusion, exhibiting a 4.6-fold increase (p < 0.05, n = 4) and 6.8-fold increase (p < 0.05, n = 4) in the ischemic cortex over control, respectively. TNF-alpha mRNA reached its peak at 3 h (8.0-fold, p < 0.05), whereas IL-1 beta mRNA reached its peak at 6 h (29.5-fold, p < 0.05). Both cytokine mRNA levels remained elevated for up to 2 d after reperfusion. In contrast to the time course of these cytokine mRNAs, c-fos and zif268 mRNAs, two early response genes, displayed a greater and earlier time-response profile. The early induction of c-fos and zif268 mRNAs in temporary brain ischemia with reperfusion suggests their roles in transcriptional regulation. The later concomitant expression of TNF-alpha and IL-1 beta suggests that these cytokines play an important role in the inflammatory response associated with focal ischemia.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
1044-7393
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:geneSymbol |
c-fos,
zif268
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
103-14
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:7702701-Animals,
pubmed-meshheading:7702701-Blotting, Northern,
pubmed-meshheading:7702701-Cerebral Cortex,
pubmed-meshheading:7702701-Gene Expression,
pubmed-meshheading:7702701-Interleukin-1,
pubmed-meshheading:7702701-Ischemic Attack, Transient,
pubmed-meshheading:7702701-Male,
pubmed-meshheading:7702701-RNA, Messenger,
pubmed-meshheading:7702701-Rats,
pubmed-meshheading:7702701-Rats, Inbred SHR,
pubmed-meshheading:7702701-Reperfusion Injury,
pubmed-meshheading:7702701-Tumor Necrosis Factor-alpha
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pubmed:articleTitle |
Concomitant cortical expression of TNF-alpha and IL-1 beta mRNAs follows early response gene expression in transient focal ischemia.
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pubmed:affiliation |
Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406.
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pubmed:publicationType |
Journal Article
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