Linked Life Data

7694540

Source:http://linkedlifedata.com/resource/pubmed/id/7694540

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5A
pubmed:dateCreated
1993-12-17
pubmed:abstractText
The influence of bovine serum albumin on estradiol-17 beta growth and responsiveness of human mammary cancer MCF-7 cells was studied and compared to that of dextran-coated charcoal-treated fetal calf serum and other bovine serum proteins. To reveal the protein factor role, a culture defined medium was used. It was composed of minimal essential medium without phenol red, added with human iron saturated tranferrin (10 mg/ml) and porcine insulin (5 mg/ml). An estradiol-17 beta mitogenic effect was noticed only in the present of bovine serum albumin or of dextran-coated charcoal-treated fetal calf serum. A protein concentration increase provokes a greater responsiveness of MCF-7 cells submitted to an estrogenic treatment. The obtained results suggest that serum albumin participates in the estradiol-17 beta mode of action on the target cells and modulates its effect. The culture conditions appear essential for the study of the effect of estrogens and antiestrogens on the growth of estrogen-dependent cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0250-7005
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1581-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Modulation of the estradiol-17 beta mitogenic effect on human breast cancer MCF-7 cells by serum albumin in defined medium.
pubmed:affiliation
Laboratoire de Biochimie, Faculté de Pharmacie, Limoges, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't