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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5133
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pubmed:dateCreated |
1993-11-18
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pubmed:databankReference |
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pubmed:abstractText |
The large subunit of the human pre-messenger RNA splicing factor U2 small nuclear ribonucleoprotein auxiliary factor (hU2AF65) is required for spliceosome assembly in vitro. A complementary DNA clone encoding the large subunit of Drosophila U2AF (dU2AF50) has been isolated. The dU2AF50 protein is closely related to its mammalian counterpart and contains three carboxyl-terminal ribonucleoprotein consensus sequence RNA binding domains and an amino-terminal arginine- and serine-rich (R/S) domain. Recombinant dU2AF50 protein complements mammalian splicing extracts depleted of U2AF activity. Germline transformation of Drosophila with the dU2AF50 complementary DNA rescues a lethal mutation, establishing that the dU2AF50 gene is essential for viability. R/S domains have been found in numerous metazoan splicing factors, but their function is unknown. The mutation in Drosophila U2AF will allow in vivo analysis of a conserved R/S domain-containing general splicing factor.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
262
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
569-73
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7692602-Amino Acid Sequence,
pubmed-meshheading:7692602-Animals,
pubmed-meshheading:7692602-Base Sequence,
pubmed-meshheading:7692602-Conserved Sequence,
pubmed-meshheading:7692602-DNA, Complementary,
pubmed-meshheading:7692602-Drosophila melanogaster,
pubmed-meshheading:7692602-Female,
pubmed-meshheading:7692602-Gene Transfer Techniques,
pubmed-meshheading:7692602-Genes, Insect,
pubmed-meshheading:7692602-Genes, Lethal,
pubmed-meshheading:7692602-In Situ Hybridization,
pubmed-meshheading:7692602-Male,
pubmed-meshheading:7692602-Molecular Sequence Data,
pubmed-meshheading:7692602-Mutation,
pubmed-meshheading:7692602-Nuclear Proteins,
pubmed-meshheading:7692602-RNA,
pubmed-meshheading:7692602-RNA Precursors,
pubmed-meshheading:7692602-RNA Splicing,
pubmed-meshheading:7692602-Recombinant Proteins,
pubmed-meshheading:7692602-Ribonucleoproteins,
pubmed-meshheading:7692602-Sequence Alignment
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pubmed:year |
1993
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pubmed:articleTitle |
The conserved pre-mRNA splicing factor U2AF from Drosophila: requirement for viability.
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pubmed:affiliation |
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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