Linked Life Data

7692369

Source:http://linkedlifedata.com/resource/pubmed/id/7692369

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1993-11-24
pubmed:abstractText
FLT4 is a recently cloned gene encoding a transmembrane tyrosine kinase related to the FLT1 and KDR/FLK1 vascular endothelial growth factor receptors. We have previously shown that FLT4 is expressed as transcripts of 4.5 and 5.8 kb in several human fetal and adult tissues. Here we show that these transcripts encode two polypeptides, FLT4s (short) and FLT41 (long), which are proteolytically processed in transfected cells and leukemia cells and which have different carboxy terminal tails. The 3' coding region of the 5.8 kb mRNA was found to be 65 codons longer than that of the the 4.5 kb mRNA. Analysis of the genomic structure of the region encoding the two carboxy termini revealed that the two transcripts are generated by alternative polyadenylation and subsequent alternative splicing during RNA processing. Our findings thus show regulation of FLT4 structure in the carboxy terminal tail considered important for receptor function. The significance of the two forms may relate to the role of additional potential autophosphorylation sites in the FLT4 long form.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:geneSymbol
FLT4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2931-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7692369-Alternative Splicing, pubmed-meshheading:7692369-Amino Acid Sequence, pubmed-meshheading:7692369-Base Sequence, pubmed-meshheading:7692369-Cloning, Molecular, pubmed-meshheading:7692369-Exons, pubmed-meshheading:7692369-Humans, pubmed-meshheading:7692369-Molecular Sequence Data, pubmed-meshheading:7692369-Proto-Oncogene Proteins, pubmed-meshheading:7692369-Proto-Oncogene Proteins c-kit, pubmed-meshheading:7692369-RNA, Messenger, pubmed-meshheading:7692369-RNA Processing, Post-Transcriptional, pubmed-meshheading:7692369-Receptor, Macrophage Colony-Stimulating Factor, pubmed-meshheading:7692369-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:7692369-Receptors, Cell Surface, pubmed-meshheading:7692369-Receptors, Colony-Stimulating Factor, pubmed-meshheading:7692369-Tumor Cells, Cultured, pubmed-meshheading:7692369-Vascular Endothelial Growth Factor Receptor-3
pubmed:year
1993
pubmed:articleTitle
Two human FLT4 receptor tyrosine kinase isoforms with distinct carboxy terminal tails are produced by alternative processing of primary transcripts.
pubmed:affiliation
Department of Pathology, University of Helsinki, Finland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't