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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1993-11-12
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pubmed:abstractText |
Past studies have shown that serum-free cultures of PC12 cells are a useful model system for studying the mechanisms of neuronal death after neurotrophic factor deprivation. These cultures, as well as NGF-deprived cultures of sympathetic neurons, manifest and endonuclease activity that leads to "apoptotic" internucleosomal DNA cleavage. Overexpression of the proto-oncogene bcl-2 blocks apoptotic death in various cell types. To study the actions of this protein in neuronal cells, we derived PC12 cell lines stably transfected with a cDNA encoding human bcl-2. It is reported here that lines expressing high levels of the exogenous bcl-2 protein are protected from both death and apoptotic DNA fragmentation caused by removal of trophic support. However, expression of high levels of exogenous bcl-2 neither mimics nor interferes with promotion of neurite outgrowth by NGF.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0270-6474
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:geneSymbol |
bcl-2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4422-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7692014-Animals,
pubmed-meshheading:7692014-Blotting, Northern,
pubmed-meshheading:7692014-Blotting, Western,
pubmed-meshheading:7692014-Cell Differentiation,
pubmed-meshheading:7692014-Cell Survival,
pubmed-meshheading:7692014-Humans,
pubmed-meshheading:7692014-Kinetics,
pubmed-meshheading:7692014-Nerve Growth Factors,
pubmed-meshheading:7692014-Neurons,
pubmed-meshheading:7692014-PC12 Cells,
pubmed-meshheading:7692014-Protein-Tyrosine Kinases,
pubmed-meshheading:7692014-Proto-Oncogene Proteins,
pubmed-meshheading:7692014-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:7692014-Proto-Oncogenes,
pubmed-meshheading:7692014-RNA,
pubmed-meshheading:7692014-RNA, Messenger,
pubmed-meshheading:7692014-Recombinant Proteins,
pubmed-meshheading:7692014-Time Factors,
pubmed-meshheading:7692014-Transfection
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pubmed:year |
1993
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pubmed:articleTitle |
Bcl-2 affects survival but not neuronal differentiation of PC12 cells.
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pubmed:affiliation |
Department of Pathology, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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