Linked Life Data

7691932

Source:http://linkedlifedata.com/resource/pubmed/id/7691932

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1993-11-24
pubmed:abstractText
We describe a method that enables detection and immunophenotypical characterization of distinct subpopulations within a cytogenetically defined tumor clone. Coexisting normal cells do not hinder microscopic evaluation because they can be distinguished from cytogenetically aberrant tumor cells. This is also true when normal and neoplastic cells cannot be clearly distinguished by cytology or immunohistochemistry, i.e., if both constituents have similar immunophenotypes and morphology. The method is based on fluorescence double staining for two different antigens combined with interphase cytogenetic analysis. It is referred to as "Fluorescence immunophenotyping and Interphase Cytogenetics as a Tool for Investigation of Neoplasms (FICTION)." In a case of follicular lymphoma we demonstrate that FICTION can differentiate bcl-2-positive malignant and non-malignant cells and can verify the presence of bcl-2-positive but cytogenetically inconspicuous T-lymphocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1554
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1641-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7691932-Antigens, CD, pubmed-meshheading:7691932-Antigens, CD20, pubmed-meshheading:7691932-Antigens, CD3, pubmed-meshheading:7691932-Antigens, Differentiation, B-Lymphocyte, pubmed-meshheading:7691932-Antigens, Neoplasm, pubmed-meshheading:7691932-Cytogenetics, pubmed-meshheading:7691932-Female, pubmed-meshheading:7691932-Fluorescent Antibody Technique, pubmed-meshheading:7691932-Humans, pubmed-meshheading:7691932-Immunophenotyping, pubmed-meshheading:7691932-In Situ Hybridization, pubmed-meshheading:7691932-Lymphocyte Subsets, pubmed-meshheading:7691932-Lymphoma, Follicular, pubmed-meshheading:7691932-Proto-Oncogene Proteins, pubmed-meshheading:7691932-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:7691932-Staining and Labeling, pubmed-meshheading:7691932-Tumor Markers, Biological
pubmed:year
1993
pubmed:articleTitle
Discrimination of distinct subpopulations within a tumor with combined double immunophenotyping and interphase cytogenetics.
pubmed:affiliation
Institut für Humangenetik, Klinikum, Christian-Albrechts-Universität, Kiel, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't