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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-8-24
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pubmed:databankReference |
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pubmed:abstractText |
PRAD1 (previously D11S287) is a putative proto-oncogene at 11q13, activated by overexpression through gene rearrangement or gene amplification in several types of human tumors including parathyroid adenomas, centrocytic lymphomas and other B-cell tumors with t(11;14), and breast cancers. PRAD1 (also CCND1) encodes cyclin D1, which may regulate the G1-S phase transition in the cell cycle. Here, we report the cloning and characterization of the chromosomal PRAD1/cyclin D1 gene and the sequence of its promoter region. The gene spans about 15 kb and has 5 exons; its promoter region has Sp1 binding sites and no obvious TATA box, characteristics of housekeeping genes and growth-regulating genes. Furthermore, an E2F binding motif present close to the major transcription start site may be involved in cell cycle-dependent expression of this gene. We also report the sequence of DNAs spanning joining regions of a reciprocal parathyroid hormone/PRAD1 gene rearrangement in a parathyroid adenoma. Comparison with normal sequences suggests that the rearrangement was not a simple break-and-ligate event, but rather involved multiple steps, including two microdeletions and a microinversion. Very short sequences conserved near the breakpoints and symmetrical elements in the eventually inverted DNA segment might have played a role in this illegitimate complex recombination, which may have similarities with a constitutional translocation in Duchenne muscular dystrophy.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1045-2257
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:geneSymbol |
CCND1,
PRAD1,
PTH
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
89-95
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7687458-Adenoma,
pubmed-meshheading:7687458-Amino Acid Sequence,
pubmed-meshheading:7687458-Base Sequence,
pubmed-meshheading:7687458-Cloning, Molecular,
pubmed-meshheading:7687458-Cyclin D1,
pubmed-meshheading:7687458-Cyclins,
pubmed-meshheading:7687458-DNA, Neoplasm,
pubmed-meshheading:7687458-Gene Rearrangement,
pubmed-meshheading:7687458-Humans,
pubmed-meshheading:7687458-Molecular Sequence Data,
pubmed-meshheading:7687458-Oncogene Proteins,
pubmed-meshheading:7687458-Parathyroid Hormone,
pubmed-meshheading:7687458-Parathyroid Neoplasms,
pubmed-meshheading:7687458-Promoter Regions, Genetic,
pubmed-meshheading:7687458-Proto-Oncogenes,
pubmed-meshheading:7687458-Sequence Homology, Nucleic Acid
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pubmed:year |
1993
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pubmed:articleTitle |
PRAD1/cyclin D1 proto-oncogene: genomic organization, 5' DNA sequence, and sequence of a tumor-specific rearrangement breakpoint.
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pubmed:affiliation |
Endocrine Unit, Massachusetts General Hospital, Boston 02114.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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