Linked Life Data

7686723

Source:http://linkedlifedata.com/resource/pubmed/id/7686723

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-8-3
pubmed:abstractText
A high-pH anion-exchange chromatography (HPAEC) system with a pulsed amperometric detector was developed for analysis of O-linked oligosaccharides in glycoproteins. Since O-linked oligosaccharides are usually released from glycoproteins as alditols by beta-elimination with reduction and they are retained less on an anion-exchange resin than their reducing counterparts, a special set of HPAEC conditions optimized for separation of sialyl O-linked oligosaccharides different from those for N-linked oligosaccharide separation had to be devised. HPAEC separation of desialylated O-linked oligosaccharides is even more difficult because of the lack of carboxylic acids or anomeric hydroxyls. Therefore, a precolumn derivatization technique was developed for these oligosaccharides. Reduced desialylated oligosaccharides were de-N-acetylated followed by N-succinylation, which endowed a negative charge at each amino sugar residue in the oligosaccharides. The desialylated oligosaccharides are derivatized in the same vial and can be easily separated on HPAEC. The new HPAEC technique presented here promises to be a valuable tool for analysis of O-linked oligosaccharides in glycoproteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
211
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
72-80
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Separation and identification of O-linked oligosaccharides derived from glycoproteins by high-pH anion-exchange chromatography.
pubmed:affiliation
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
pubmed:publicationType
Journal Article