Linked Life Data

7681398

Source:http://linkedlifedata.com/resource/pubmed/id/7681398

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1993-4-23
pubmed:databankReference
pubmed:abstractText
We determined the nucleotide sequence of the VL and VH regions of four human monoclonal IgM directed to myelin-associated glycoprotein (MAG) and a nerve glycolipid, the sulfated glucuronic paragloboside (SGPG). Clonal lymphoblastoid cell lines (three cases) and an heterohybridoma (one case) secreting anti-MAG IgM were derived from patients' blood B cells. V kappa genes derived from the single germinal V kappa IV (two cases), the V kappa Id and the V kappa IIIa Humkv328h5 genes. VH genes derived from the VHIII 9.1 germinal gene (or a closely related gene) in two cases, whereas two others possibly represent new members of the VHIII or VHI variability subgroups. There was no obvious restriction in the use of J kappa, JH and DH segments. Somatic mutations were predominantly found in the CDR3 of the V kappa IV genes with an overall ratio of replacement over silent mutations of 7/0. The sequence of two VHIII genes exhibited five replacement mutations in CDR in comparison to that of the germ-line 9.1 gene. Although some V genes are likely to be overrepresented among anti-MAG IgM, the diversity of the immune repertoire for MAG and SGPG explains the lack of easily detectable public idiotopes among these IgM. This last finding, as well as a high ratio of replacement versus silent nucleotide mutations in the CDR of VL and probably VH genes, suggest that the pathogenesis of these monoclonal antibodies (and of the associated lymphoplasmocytic disorder) differs from that of other previously characterized monoclonal autoantibodies such as rheumatoid factors and cold agglutinins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
846-51
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7681398-Amino Acid Sequence, pubmed-meshheading:7681398-Antibodies, Monoclonal, pubmed-meshheading:7681398-Base Sequence, pubmed-meshheading:7681398-Clone Cells, pubmed-meshheading:7681398-Genes, Immunoglobulin, pubmed-meshheading:7681398-Glycoproteins, pubmed-meshheading:7681398-Humans, pubmed-meshheading:7681398-Immunoglobulin M, pubmed-meshheading:7681398-Immunoglobulin Variable Region, pubmed-meshheading:7681398-Immunoglobulin kappa-Chains, pubmed-meshheading:7681398-Immunoglobulin mu-Chains, pubmed-meshheading:7681398-Molecular Sequence Data, pubmed-meshheading:7681398-Myelin Proteins, pubmed-meshheading:7681398-Myelin-Associated Glycoprotein, pubmed-meshheading:7681398-Nerve Tissue Proteins, pubmed-meshheading:7681398-Oligodeoxyribonucleotides, pubmed-meshheading:7681398-Sequence Alignment
pubmed:year
1993
pubmed:articleTitle
Nucleotidic sequence analysis of the variable domains of four human monoclonal IgM with an antibody activity to myelin-associated glycoprotein.
pubmed:affiliation
Laboratory of Immunopathology, Hôpital Saint-Louis, Paris, France.
pubmed:publicationType
Journal Article, Comparative Study