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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1993-4-6
|
| pubmed:databankReference | |
| pubmed:abstractText |
We describe the genomic structure of the rat TRPM-2 gene. This gene is induced de novo during the regression of the prostate and other hormone-dependent tissues after hormone ablation and plays an important role in apoptosis, or active cell death. TRPM-2 is a single copy gene, organized into nine exons, ranging in size from 47 base pairs (exon I) to 412 base pairs (exon IV), spanning a region of 13,750 base pairs. Comparison with sequences registered in the data bases shows that it has extensive homology to SGP-2, a gene that is expressed constitutively in the testis, although the sequences diverge dramatically in the 5'-untranslated region of the mRNA (coded for by exon I in TRPM-2), raising the possibility of alternative exon I usage in SGP-2. Primer extension, S1 nuclease protection, and extensive polymerase chain reaction analysis suggest that the TRPM-2 transcript from the prostate and the SGP-2 transcript from the testis are in fact identical and only contain the exon I sequence identified in the TRPM-2 genomic clone. Analysis of the promoter region of the TRPM-2 gene demonstrates that the putative control region contains several potential regulatory elements that may regulate the complex tissue-specific control of a gene which must be constitutively expressed in some tissues but repressed in others until induced during active cell death.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Clu protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Clusterin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Molecular Chaperones,
http://linkedlifedata.com/resource/pubmed/chemical/RNA
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
268
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
5021-31
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:7680346-Amino Acid Sequence,
pubmed-meshheading:7680346-Animals,
pubmed-meshheading:7680346-Base Sequence,
pubmed-meshheading:7680346-Blotting, Southern,
pubmed-meshheading:7680346-Cell Death,
pubmed-meshheading:7680346-Clusterin,
pubmed-meshheading:7680346-DNA,
pubmed-meshheading:7680346-Exons,
pubmed-meshheading:7680346-Gene Expression Regulation,
pubmed-meshheading:7680346-Glycoproteins,
pubmed-meshheading:7680346-Male,
pubmed-meshheading:7680346-Molecular Chaperones,
pubmed-meshheading:7680346-Molecular Sequence Data,
pubmed-meshheading:7680346-Orchiectomy,
pubmed-meshheading:7680346-Promoter Regions, Genetic,
pubmed-meshheading:7680346-RNA,
pubmed-meshheading:7680346-Rats,
pubmed-meshheading:7680346-Sequence Homology, Nucleic Acid,
pubmed-meshheading:7680346-Transcription, Genetic
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Genomic organization and expression of the rat TRPM-2 (clusterin) gene, a gene implicated in apoptosis.
|
| pubmed:affiliation |
Department of Biochemistry, University of Ottawa, Ontario, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|