Linked Life Data

7678795

Source:http://linkedlifedata.com/resource/pubmed/id/7678795

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-3-2
pubmed:databankReference
pubmed:abstractText
Insulin-degrading enzyme (IDE), a cytosolic metalloendoprotease, can degrade insulin, insulin-like growth factor-II, insulin-like growth factor-I, and transforming growth factor-alpha. While IDE has been implicated in the cellular degradation of insulin, other physiological functions of this enzyme are not known. To assess the possible role of IDE in cellular growth and development, we determined the tissue and developmental distribution of the enzyme. Rat IDE cDNA fragments and antibodies directed against human IDE were used to probe IDE transcripts and proteins in rat tissues. The results demonstrate that IDE transcripts are ubiquitous in rat tissues. The level of rIDE transcripts is high in adult rat testis, tongue, and brain; moderate in kidney, prostate, heart, muscle, liver, intestine, and skin; and low in spleen, lung, thymus, and uterus. The sizes of the major transcripts of rIDE are 3.4 and 6.3 kilobases in all tissues analyzed, except testis. Surprisingly, the highest level of rIDE mRNA in the adult rat was in the testis, and the major transcripts of rIDE in this tissue were shifted in size to 3.8 and 6.7 kilobases. Immunocytochemical analysis localized the rIDE mainly in the epithelium of prostate gland and kidney, and the cytosol of liver hepatocytes. During rat development from 6-7 days of age to adulthood, rIDE mRNA levels increased in brain, testis, and tongue; decreased in muscle and skin; and did not significantly change in other tissues examined. These studies reveal regulation of IDE or IDE-related genes in rat tissues and during rat development, suggesting that this enzyme may have multiple functions relating to cellular growth and development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
132
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
604-11
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7678795-Aging, pubmed-meshheading:7678795-Amino Acid Sequence, pubmed-meshheading:7678795-Animals, pubmed-meshheading:7678795-Base Sequence, pubmed-meshheading:7678795-Blotting, Northern, pubmed-meshheading:7678795-Brain, pubmed-meshheading:7678795-DNA, pubmed-meshheading:7678795-Gene Expression Regulation, Enzymologic, pubmed-meshheading:7678795-Gene Library, pubmed-meshheading:7678795-Humans, pubmed-meshheading:7678795-Immunohistochemistry, pubmed-meshheading:7678795-Insulysin, pubmed-meshheading:7678795-Kidney, pubmed-meshheading:7678795-Liver, pubmed-meshheading:7678795-Male, pubmed-meshheading:7678795-Molecular Sequence Data, pubmed-meshheading:7678795-Organ Specificity, pubmed-meshheading:7678795-Prostate, pubmed-meshheading:7678795-RNA, pubmed-meshheading:7678795-Rats, pubmed-meshheading:7678795-Rats, Sprague-Dawley, pubmed-meshheading:7678795-Sequence Homology, Amino Acid, pubmed-meshheading:7678795-Sequence Homology, Nucleic Acid, pubmed-meshheading:7678795-Transcription, Genetic
pubmed:year
1993
pubmed:articleTitle
Insulin-degrading enzyme is differentially expressed and developmentally regulated in various rat tissues.
pubmed:affiliation
Ben May Institute, University of Chicago, Illinois 60637.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't