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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5230
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pubmed:dateCreated |
1995-10-12
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pubmed:abstractText |
The mating response pathway of the yeast Saccharomyces cerevisiae includes a heterotrimeric guanine nucleotide-binding protein (G protein) that activates a mitogen-activated protein MAP kinase cascade by an unknown mechanism. An amino-terminal fragment of the MAP kinase scaffold protein Ste5p that interfered with pheromone-induced cell cycle arrest was identified. A haploid-specific interaction between the amino terminus of Ste5p and the G protein beta subunit Ste4p was also detected in a two-hybrid assay, and the product of a signaling-defective allele of STE4 was defective in this interaction. In cells with a constitutively activated pheromone response pathway, epitope-tagged Ste4p was coimmunoprecipitated with Ste5p. Thus, association of the G protein and the MAP kinase cassette via the scaffolding protein Ste5p may transmit the G protein signal.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein beta Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein gamma Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Heterotrimeric GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Pheromones,
http://linkedlifedata.com/resource/pubmed/chemical/STE18 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/STE5 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ste4 protein, S cerevisiae
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
269
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pubmed:geneSymbol |
STE4,
STE5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1572-5
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7667635-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:7667635-Base Sequence,
pubmed-meshheading:7667635-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:7667635-Carrier Proteins,
pubmed-meshheading:7667635-Cell Division,
pubmed-meshheading:7667635-Fungal Proteins,
pubmed-meshheading:7667635-GTP-Binding Protein beta Subunits,
pubmed-meshheading:7667635-GTP-Binding Protein gamma Subunits,
pubmed-meshheading:7667635-GTP-Binding Proteins,
pubmed-meshheading:7667635-Heterotrimeric GTP-Binding Proteins,
pubmed-meshheading:7667635-Molecular Sequence Data,
pubmed-meshheading:7667635-Mutation,
pubmed-meshheading:7667635-Pheromones,
pubmed-meshheading:7667635-Plasmids,
pubmed-meshheading:7667635-Saccharomyces cerevisiae,
pubmed-meshheading:7667635-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:7667635-Signal Transduction,
pubmed-meshheading:7667635-Transformation, Genetic
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pubmed:year |
1995
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pubmed:articleTitle |
Association of the yeast pheromone response G protein beta gamma subunits with the MAP kinase scaffold Ste5p.
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pubmed:affiliation |
Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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